Micro-fluidic chip for nucleic acid detection and nucleic acid detection device

Abstract

The invention provides a micro-fluidic chip for nucleic acid detection and a nucleic acid detection device. The micro-fluidic chip comprises a sample purification cavity, an amplification liquid preparation cavity, an amplification liquid retention cavity, a mixing flow channel, a reaction liquid collection cavity, a liquid separation structure, amplification reaction cavities and a waste liquid cavity, wherein under first centrifugal motion, amplification liquid in the amplification liquid preparation cavity can flow into the amplification liquid retention cavity, and sample liquid in the sample purification cavity is retained in the sample purification cavity under the action of capillary force; under second centrifugal motion, the amplification liquid and the sample liquid can break through the constraint of the capillary force to enter the mixing flow channel, and are fully mixed and then converged in the reaction liquid collection cavity; and under third centrifugal motion, reaction liquid in the reaction liquid collection cavity is uniformly distributed to the amplification reaction cavities through the liquid separation structure, so that constant-temperature amplification reaction and real-time fluorescence detection can be conveniently carried out in the amplification reaction cavities. The micro-fluidic chip can realize quick, simple and stable centrifugal liquid separation operation, and conveniently, efficiently and reliably complete the constant-temperature amplification reaction and the fluorescence detection.

Description

technical field [0001] The invention relates to the field of molecular diagnosis, in particular to a microfluidic chip and nucleic acid detection equipment for nucleic acid detection. Background technique [0002] Traditional nucleic acid detection methods include sample collection, nucleic acid extraction, nucleic acid amplification and detection, relying on fluorescent quantitative polymerase chain reaction (PCR) technology, requiring laboratory conditions of biosafety level 2 and above and biosafety level 3 experiments Laboratory-level personal protection largely limits testing to institutions such as high-level CDCs and tertiary hospitals. Relatively limited medical resources face a large number of people to be tested, and traditional nucleic acid detection methods have gradually exposed many defects: the nucleic acid extraction process is cumbersome and expensive, the cost of reagents is high, the detection time is long, the ability to process a large number of samples ...

AI Technical Summary

Problems solved by technology

Relatively limited medical resources face a large number of people to be tested, and traditional nucleic acid detection methods have gradually exposed many defects: the nucleic acid extraction process is cumbersome and expensive, the cost of reagents is high, the detection time is long, the ability to process a large number of samples is weak, the level of automation is low, and the degree of integration is poor , requires professional testing personnel and expensive equipment, and has been unable to meet the new needs of high-standard, high-quality, high-efficiency nucleic acid testing under the new situation

At the same time, the virus is highly contagious, and aerosol pollution is easily formed during the nucleic acid extraction and amplification process, which puts forward new requirements for the protection of medical personnel and poses a potential threat to the health of testing personnel

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