A method of stabilizing carbidopa/levodopa in a biological matrix
A biological matrix and levodopa technology, applied in the biological field, can solve the problems of not being able to truly reflect the drug situation, and achieve the effect of reducing enzymatic reactions, reducing enzymatic reactions, and inhibiting oxidation reactions
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0024] Measure 9 mL of sterile water for injection to dissolve completely, and then add 1000 μL of formic acid to obtain stabilizer 10% FA;
[0025] Add 25 μL of stabilizer 10% FA to the pre-cooled EP tube, then add 0.975 mL of plasma, and add 100 μL of 0.03 mg / mL CD / LD after mixing. Take an appropriate amount of the first sample immediately for quantitative detection, the second sample is placed in a wet ice environment for 3 hours for quantitative detection, and the third sample is placed in a wet ice environment for 20 hours for quantitative detection. The results are shown in Table 1 and Table 2.
Embodiment 2
[0027] Take 8 mL of sterile water for injection to dissolve completely, and then add 2000 μL of formic acid to obtain stabilizer 20% FA;
[0028] Add 25 μL of stabilizer 20% FA to the pre-cooled EP tube, then add 0.975 mL of plasma, and add 100 μL of 0.03 mg / mL CD / LD after mixing. Take an appropriate amount of the first sample immediately for quantitative detection, the second sample is placed in a wet ice environment for 3 hours for quantitative detection, and the third sample is placed in a wet ice environment for 20 hours for quantitative detection. The results are shown in Table 1 and Table 2.
Embodiment 3
[0030] Measure 9.5 mL of sterile water for injection, and then add 500 μL of trifluoroacetic acid to obtain stabilizer 5% TFA;
[0031] Add 25 μL of stabilizer 5% TFA to the pre-cooled EP tube, then add 0.975 mL of plasma, and add 100 μL of 0.03 mg / mL CD / LD after mixing. Take an appropriate amount of the first sample immediately for quantitative detection, the second sample is placed in a wet ice environment for 3 hours for quantitative detection, and the third sample is placed in a wet ice environment for 20 hours for quantitative detection. The results are shown in Table 1 and Table 2.
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More