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Hypocotyl grafting method for interspecific interaction research

A technology of hypocotyl and grafting clip, applied in the field of hypocotyl grafting, to achieve the effect of improving the survival rate, reducing the inhibition, and the grafted seedlings being completely consistent

Pending Publication Date: 2021-10-01
NINGBO ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of this invention is to provide a kind of hypocotyl grafting method that is used for interspecific interaction research, to solve the above problems

Method used

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  • Hypocotyl grafting method for interspecific interaction research
  • Hypocotyl grafting method for interspecific interaction research
  • Hypocotyl grafting method for interspecific interaction research

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Using cucumber stock to graft cucumbers to carry out low-temperature-resistant stock-ear interaction research experiments. The low-temperature-resistant cucumber stock variety Huangzhen No. 2, which was bred by Ningbo Academy of Agricultural Sciences and sold by Ningbo Fengdeng Seed Industry Technology Co., Ltd., was used as rootstock 1. Rootstock 1 Material Huangzhen No. 2 and the relatively low-temperature-resistant fruit cucumber variety Bicui 20 purchased from Zhejiang Wuwangnong Seed Industry Co., Ltd. were scions 2, which were grafted by hypocotyl grafting; Huangzhen No. 2 rootstock was passed Two Huangzhen 2 scion 2 were grafted by hypocotyl grafting method; Huangzhen 2 was used as rootstock 1 to graft Bicui 20 and Bicui 20 was used as rootstock 1 to graft two Bicui 20 scion 2 as a control to study the relationship between the two materials. Low temperature tolerance interaction.

[0037] Proceed as follows:

[0038] 1.1. Grafting and raising seedlings

[0039]...

Embodiment 2

[0056] Embodiment 2, utilizing India-China hybrid pumpkin type stock 1 to graft two muskmelons of different colors

[0057] Selected by Ningbo Academy of Agricultural Sciences and sold by Ningbo Fengdeng Seed Industry Technology Co., Ltd. India-China hybrid pumpkin type rootstock 1 Sizhuang No. 7 as the rootstock, white-skinned orange-fleshed melon Yongtian No. 5, green-skinned orange-fleshed melon Fengdeng honey 25 is the scion 2, which is produced by grafting by the hypocotyl grafting method.

[0058] Proceed as follows:

[0059] 1.1 Grafting and raising seedlings

[0060] In the autumn of 2020, grafting seedlings will be carried out in the seedling greenhouse of Ningbo High-tech Agricultural Technology Experimental Park.

[0061] Select 1 seed of pumpkin rootstock that is plump and consistent, sterilize the seed by scalding it in hot water at 70°C for 15 minutes, stirring constantly, after soaking the seed, wash the seed, and then soak it in clean water at room temperatur...

Embodiment 3

[0072] Using Ningbo Academy of Agricultural Sciences to select and breed, the gourd-type rootstock Yongzhen No. 1 sold by Ningbo Fengdeng Seed Industry Technology Co., Ltd. was grafted with watermelon Zaojia purchased from Zhejiang Wuwangnong Seed Industry Co., Ltd., and Yongzhen No. 1 was respectively set as Rootstock 1 double-grafted Yongzhen No. 1, Yongzhen No. 1 was rootstock 1 single-grafted Zaojia, Zaojia was rootstock 1 double-grafted Zaojia, a total of three groups were compared, and Yongzhen No. 1 was used as rootstock 1 double-grafted Yongzhen No. 1 After surviving grafting, He Zaojia was placed in an artificial climate box for low temperature treatment at 4°C and normal temperature control. After 2 days of treatment, the leaves of Yongzhen 1 and Zaojia as scion 2 were taken respectively to investigate the malondialdehyde (MDA) content. Seedling cultivation before grafting and management after grafting are all the same as in Example 1. Single rootstock grafting singl...

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Abstract

The invention relates to a hypocotyl grafting method for interspecific interaction research. The hypocotyl grafting method comprises the following steps of cultivating scions and stocks, grafting hypocotyls, managing after grafting and the like. Compared with the prior art, improvement points comprise that the stock is cut, the scion is grafted in a joint grafting mode after being obliquely cut, after the scion is cut, the cut is soaked in a mixed solution with the main components of 6-benayl aminopurine, brassinolide and a cucurbita kangua leach liquor for 1-3 seconds, then the cut scion is grafted to the stock, a grafting clamp is used for clamping, and the stock and the scion are fixed. The hypocotyl grafting method which can be used for realizing interspecific interaction research is provided for the first time, and the method can further be applied to grafted seedling production of seedling raising enterprises, so that the morbidity of plants is reduced, the labor intensity is reduced, the survival rate is increased, and the seedling raising time is shortened.

Description

technical field [0001] The invention relates to a method for grafting hypocotyls used for the study of interspecific interactions. Background technique [0002] Grafting is an effective green cultivation technique to solve continuous cropping obstacles such as soil-borne diseases, improve plant stress tolerance, and improve water and fertilizer utilization efficiency. Grafting can not only regulate plant growth, flowering period, water and fertilizer absorption capacity, and photosynthesis, but also regulate hormone balance, nutrient absorption, synthesis and transport, and there are molecular exchanges such as RNA and protein between rootstock and ear. [0003] Most of the existing grafting methods leave rootstock cotyledons, which are easy to germinate new sprouts of the rootstock during the grafting process. At the same time, most of the existing grafting methods are one rootstock grafted with one scion, and in the research process, the rootstock-scion interaction can onl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G2/30
CPCA01G2/30
Inventor 邢乃林张蕾琛严蕾艳古斌权王迎儿黄芸萍王毓洪马二磊张华峰
Owner NINGBO ACAD OF AGRI SCI
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