Hydroxyapatite and collagen composition and application thereof in nevus removing, freckle removing, pigmentation reducing and whitening
A technology of hydroxyapatite and collagen, which is applied in the fields of medical devices, cosmetics, and medicine, can solve problems such as disfigurement, pigmentation, and depigmentation.
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Embodiment 1
[0103] To explore the effect of different proportions of HAP and collagen combined on the synthesis of melanin in B16 melanoma cells.
[0104] Determination of melanin content: B16 cells in the logarithmic growth phase were digested with 0.25% trypsin, and the cell concentration was adjusted to 5×10 5 / ml, inoculate in 6-well culture plate, add 2ml of cell suspension to each well, place at 37°C, 5% CO 2 cultured in an incubator. After the cells adhered to the wall for 24 h, the culture medium was discarded. In the experimental group, 2 ml of the whole culture solution containing HAP and collagen was added according to the concentrations shown in Table 1, and there were 81 groups in total.
[0105] Table 1
[0106]
[0107] An equal amount of complete medium was added to the blank group. Each group of treatments replicated 6 wells. Add 1 mol / L NaOH containing 10% DMSO, sonicate for 30 min, place in a 90° C. water bath for 2 h, and measure the absorbance (A) at 450 nm. ...
Embodiment 2
[0119] Mole Lightening / Mole Removal
[0120] 1. Evaluation method:
[0121] Select a healthy adult, where there are melanomas growing, select a 2cm*2cm test area, and count the number of melanomas in the test area.
[0122] Distribute the test product, use it twice a day, once in the morning and once in the evening, and use it after cleansing. Stop using other mole lightening and mole removal products during the experiment.
[0123] Evaluation after continuous use. Using self-contrast before and after, doctors and patients will jointly evaluate according to the following grading standards.
[0124] 0: melanoma remains, protruding from the skin surface, and the color has not changed;
[0125] 1: The nevus shrinks and protrudes from the skin surface (or the nevus does not become smaller and the protrusion decreases), and the color does not change;
[0126] 2: The color of melanoma becomes lighter or changes from black to yellowish brown;
[0127] 3: The melanoma shrinks,...
Embodiment 3
[0135] Lighten spots / freckles, reduce pigmentation
[0136] 1. Evaluation method:
[0137] Choose a healthy adult with spots or pigmentation, take a 2cm*2cm area as the test site, distribute the test product, use it twice a day, once in the morning and once in the evening, and use it after cleansing. During the experiment, stop using other spot-lightening and pigmentation-reducing products. Evaluate according to the degree of skin tone loss.
[0138] Evaluation after continuous use. Using self-contrast before and after, doctors and patients will jointly evaluate according to the following grading standards.
[0139] 0: There is no change in the area of spots and pigmentation, and there is no change in color;
[0140] 1: The area of spots and pigmentation shrinks, and the color does not change;
[0141] 2: There is no obvious change in the area of spots and pigmentation, and the color becomes lighter or changes from black to yellowish brown;
[0142] 3: The area of ...
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