A method for evaluating the effect of sweet potato black spot disease on potato seedlings at the seedbed stage

A technology for black spot and sweet potato, which is applied in the field of plant disease prevention and control effect evaluation, can solve problems such as differences in potato seedling growth, differences in disease resistance, and inability to determine the emergence of tubers, and achieves the effect of low implementation threshold.

Active Publication Date: 2022-07-19
INST OF PLANT PROTECTION HENAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the investigation of potato seedlings at the seedbed stage is still simply investigating the growth and disease of potato seedlings in the aboveground, or pulling out potato seedlings to investigate the disease.
This method cannot determine the emergence of potato pieces, as well as the growth and disease of potato seedlings before they emerge from the soil; when there are differences in the number of seedlings or disease susceptibility rates between different varieties or different control measures, it is impossible to determine the Is there a difference in the emergence of potato tubers, or is there a difference in the resistance of potato seedlings of various varieties to black spot disease; it is not sure whether different control measures have an impact on the emergence of potato tubers, or cause differences in the growth of potato tubers , and whether it caused the difference in susceptibility of potato seedlings
The shortcomings of existing survey methods have greatly interfered with the evaluation of disease resistance of resistant varieties and the effect of control measures

Method used

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  • A method for evaluating the effect of sweet potato black spot disease on potato seedlings at the seedbed stage
  • A method for evaluating the effect of sweet potato black spot disease on potato seedlings at the seedbed stage
  • A method for evaluating the effect of sweet potato black spot disease on potato seedlings at the seedbed stage

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The investigation result that embodiment 1 does not divide into nursery pond

[0028] 1. Materials and methods

[0029] 1.1 Test material

[0030] The sweet potato variety was Shangshu 19 virus-free seed potato, and non-invasive, disease-free and uniform-sized potato pieces were selected for use before the test. The medicament can be obtained by dilution with a fungicide or other medicament useful in reducing the occurrence of black spot. In this example, the test agent selected for use is SY-01, the control agent is 500 g / L isobacturon suspending agent, 70% thiophanate-methyl wettable powder, and the blank is clear water.

[0031] 1.2 Seedbed Setup

[0032] The seedbed was set in a plastic greenhouse, and the sweet potato black spot was inoculated by artificial inoculation, and the inoculation amount was 2.5 × 10 5 Spores / cm 3 , See test methods for nursery pond settings.

[0033] 2 Test methods

[0034] 2.1 Processing settings

[0035] The test plot is set up ...

Embodiment 2

[0051]Embodiment 2 The investigation result that the nursery pond is divided into zones

[0052] 1. Materials and methods

[0053] 1.1 Test material

[0054] The sweet potato variety was Shangshu 19 virus-free seed potato, and non-invasive, disease-free and uniform-sized potato pieces were selected for use before the test. The test agent is SY-01, the control agent is 500 g / L isocyanide suspension, 70% thiophanate-methyl wettable powder, and the blank control is clear water.

[0055] 1.2 Seedbed Setup

[0056] The seedbed was set in a plastic greenhouse, and the sweet potato black spot was inoculated by artificial inoculation, and the inoculation amount was 2.5 × 10 5 Spores / cm 3 , See test methods for nursery pond settings.

[0057] 2 Test methods

[0058] 2.1 Processing settings

[0059] The size of the nursery pond was 1m×1m, which was divided into an individual investigation area (1m×30cm) and a conventional investigation area (1m×70cm). The seed potatoes in the ind...

Embodiment 3

[0092] Example 3 Investigation on the emergence of different varieties of sweet potato and the incidence of black spot disease under the same inoculation conditions

[0093] 1. Materials and methods

[0094] 1.1 Test material

[0095] All varieties of potato chips are required to be non-invasive, disease-free, and uniform in size. The sweet potato varieties were Shangshu 19, Yushu 12, Zhenghong 22, Yanshu 25, and Yushu 10.

[0096] 1.2 Seedbed Setup

[0097] The seedbed was set in a plastic greenhouse, and the sweet potato black spot fungus was inoculated by artificial inoculation, and the inoculation amount was 2 × 10 5 Spores / cm 3 , See test methods for nursery pond settings.

[0098] 2 Test methods

[0099] 2.1 Processing settings

[0100] A 1m×1m nursery pond was set up and divided into a separate survey area (1m×30cm) and a conventional survey area (1m×70cm). The separate survey area and the conventional survey area were separated by 5cm for seed potatoes. A total...

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Abstract

The invention belongs to the technical field of evaluation of plant disease prevention and control effects, and discloses a method for evaluating the effect of sweet potato black spot disease on potato seedlings at the seedbed stage, comprising the following steps: picking out the sweet potatoes without trauma, disease and uniform size, and weighing to obtain Sweet potato fresh weight; set up a nursery pond, and divide the nursery pond into a separate investigation area and a conventional investigation area, and inoculate the seedbed soil with sweet potato black spot by artificial inoculation; put the sweet potato flat into the seedling pond, and cover the sweet potato with the seedbed soil, Each nursery pond is independently irrigated and managed, covered with plastic film after irrigation, and removed after emergence; the growth of potato seedlings to 5-6 leaf stage is the standard for seedlings The evaluation is divided into three parts: the investigation of mature seedlings, the investigation of undeveloped seedlings and the detection of black spot disease. The invention solves the problem that the germination of potato pieces and the immature seedlings cannot be assessed to be affected by black spot disease in the past.

Description

technical field [0001] The invention belongs to the technical field of plant disease prevention and control effect evaluation, and relates to a method for evaluating the influence of sweet potato black spot disease on potato seedlings at the seedbed stage. Background technique [0002] Sweet potato black spot occurs in all sweet potato producing areas of the world, and is one of the main diseases of sweet potato production in my country. The annual loss of sweet potato yield caused by the disease is 5%-10%, and when the damage is severe, the loss is 20%-50%, or even higher. [0003] Sweet potato can be infected with black spot throughout the growth and storage stages. In the seedling stage, when the seed potato is contaminated with fungus or the soil of the seedbed is contaminated with black spot fungus, it can lead to the occurrence of black spot disease in the seedbed stage, resulting in dead seedlings and diseased potato seedlings. Dead seedlings will reduce the emergen...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G22/25A01G7/00
CPCA01G22/25A01G7/00
Inventor 张德胜张振臣王爽王永江田雨婷赵付枚乔奇
Owner INST OF PLANT PROTECTION HENAN ACAD OF AGRI SCI
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