Dissolving method for waxy substances on coccid of family Margarodidae

Abstract

The invention provides a dissolving method for waxy substances on coccid of the family Margarodidae. According to the method, wax substances on female coccid of the family Margarodidae are dissolved through wax opening water, the female coccid is soaked in the wax opening water, heating is cooperatively conducted to enable the wax substances to be dissolved in an accelerated mode, and then proper vibration is conducted to allow the wax opening water to make full contact with the wax substances on the female coccid, so wax removal effect is achieved more quickly. The method is simple, convenient, time-saving and safe, easily removes wax protrusions and the wax substances on the female coccid, and lays a foundation for subsequent preparation of clear, complete and high-quality scale insect slide specimens.

Description

technical field [0001] The invention belongs to the technical field of biological specimen preparation, and in particular relates to a method for dissolving waxy matter of scale insects of the family Coccidae. Background technique [0002] Scale insects belong to Hemiptera (Hemiptera), Coccoidea (Coccoidea). According to the statistical report of scale insect net, there are more than 8,000 species of scale insects recorded in the world at present; there are 1,155 species of scale insects in 14 families, 302 genera and 1,155 species recorded in my country. Scale insects are mostly small in size and live in hiding. The insects are often covered with scale or waxy secretions for protection, and they are dioecious. Vitality and fecundity are extremely strong, and it is easy to erupt into disasters. Most species of scale insects are important agricultural and forestry pests. They often gather on the surface of plants and suck juice to cause damage, causing branches and leaves t...

AI Technical Summary

Problems solved by technology

Wu Bowen emphasized that the quality of the specimen plays a decisive role in the experimental results in "Study on the Taxonomy of Coccidae Insects in China (Hemiptera: Coccoides)", but due to the different sizes of Coccoids, the texture between genera and species The variation is large, and the success rate of specimen preparation is low. The key influencing factor is the waxy substance on the insect body, especially the female of Coccidae is the most difficult to deal with

[0004] The body of female Coccidae is often covered with long wax process or thick waxy secretion, such as image 3 , Figure 4 These waxy protrusions or waxy secretions are extremely difficult to remove as indicated by

[0005] Manual direct picking takes a lot of time, the operation is difficult, and the requirements for operators are also high, and the effect is not ideal. At the same time, it is very easy to damage the setae on the surface of the insect body, which will affect the production and observation of slides in the later stage.

Traditional chemical removal mainly uses carbon tetrachloride, chloroform, phenol and other drugs, which cannot completely remove all waxy substances, and the use of carbon tetrachloride is strictly restricted by the country, and it is only used for non-ozone-depleting substances raw materials Uses and special purposes; chloroform is narcotic and has the possibility of carcinogenicity, and is controlled by the public security department according to the "Regulations on the Safety Management of Hazardous Chemicals" and "Regulations on the Management of Precursor Chemicals"; phenol has been approved by the World Health Organization International Cancer Research The list of carcinogens released by the agency is a preliminary reference. Phenol is in the list of three types of carcinogens, which is more toxic to human body, unsafe, and not environmentally friendly.

In addition, traditional chemical removal methods often require overnight treatment, which takes a long time

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