Porphyrin lipid-perfluorocarbon nano preparation as well as preparation method and application thereof
A perfluorocarbon and porphyrin lipid technology, which is applied in the field of biomedical materials, can solve the problems of low perfluorocarbon loading capacity, light/acoustic drug aggregation, low generation capacity concentration, etc. Enhanced fluorescence/CT imaging and reduced toxicity and side effects
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[0037] Example 1
[0038] The preparation method of a kind of porphyrin lipid-perfluorooctane bromide nano preparation of the present invention comprises the following steps:
[0039] 1) The porphyrin lipid A (wherein R 1 and R 2 All are C16 alkyl; R 3 is hydroxyl; a=2, b=2; X is NH) (10mmol, PGL lipid) was dissolved in a mixed solvent of chloroform and methanol (volume ratio 9:1), the liquid was dried under reduced pressure to form a thin film, and the vacuum dry overnight;
[0040] 2) Add ultrapure water, hydrate for 30 min in a 60°C water bath, and then use a sonicator to sonicate under ice-bath conditions, so that the lipids are uniformly dispersed in the aqueous solution;
[0041] 3) Add perfluorooctane (350 μL, PFOB) dropwise to the solution in step 2) under ice bath conditions, and use the probe to ultrasonically disperse for 5 min to obtain uniformly dispersed porphyrin lipid-perfluorooctane The alkane nanoformulations can be further removed by centrifugation (100...
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[0043] Example 2
[0044] The preparation method of a kind of porphyrin lipid-perfluorohexane nano preparation of the present invention comprises the following steps:
[0045] 1) The porphyrin lipid B containing hematoporphyrin photo / acoustic groups (wherein R 1 and R 2 All are C12 alkyl; R 3 is polyethylene glycol; a=2, b=2; X is O) (9.5 mmol) and DSPE-mPEG2000 (0.5 mmol) were dissolved in a mixed solvent of chloroform and methanol (volume ratio 9:1) to reduce Press-spin the liquid to form a thin film, and vacuum dry overnight;
[0046] 2) Add ultrapure water, hydrate for 10 min in a water bath at 45°C, and then use a sonicator to ultrasonically treat it in an ice bath, so that the lipids are uniformly dispersed in the aqueous solution;
[0047] 3) Add perfluorohexane (330 μL) dropwise to the solution in step 2) under ice bath conditions, and use the probe to ultrasonically disperse for 10 min to obtain a uniformly dispersed porphyrin lipid-perfluorohexane nano-formulatio...
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[0048] Example 3
[0049] The preparation method of a kind of porphyrin lipid-perfluorohexane nano preparation of the present invention comprises the following steps:
[0050] 1) The porphyrin lipid C containing protoporphyrin photo / acoustic groups (wherein R 1 and R 2 All are C18 alkyl; R 3 is polyethylene glycol; a=3, b=3; X is O) (0.5 mmol), DPPC (9.0 mmol) and DSPE-Maleimide (0.5 mmol) dissolved in chloroform and methanol (9:1 by volume) In the mixed solvent of , the liquid was spin-dried under reduced pressure to form a thin film, and vacuum dried overnight;
[0051] 2) Add ultrapure water, hydrate for 20 min in a water bath at 50°C, and then use a sonicator to ultrasonically treat it in an ice bath, so that the lipids are uniformly dispersed in the aqueous solution;
[0052] 3) Add perfluorohexane (352 μL) dropwise to the solution in step 2) under ice bath conditions, and use the probe to ultrasonically disperse for 10 min to obtain a uniformly dispersed porphyrin li...
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