WAYNE293 LVPRO cell adapted to serum-free culture medium environment and application of WAYNE293 LVPRO cell

A WAYNE293, host cell technology, applied in the field of cells, can solve problems such as cell clumping, affecting cell viability, gene and cell therapy product safety risks, etc.

Active Publication Date: 2021-11-05
QUACELL BIOTECHNOLOGY CO LTD
View PDF4 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Adherent culture has insurmountable difficulties in the late stage of industrialization, because the number of cells is limited by the surface area of ​​the culture flask, so in the process of large-scale industrial production, it is necessary to expand the production scale of viral vectors, and the investment in equipment and Artificial will greatly increase the cost of virus vector preparation. At the same time, since the adherent culture process still needs to add animal-derived components of fetal bovine serum as a necessary cell culture ra

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • WAYNE293 LVPRO cell adapted to serum-free culture medium environment and application of WAYNE293 LVPRO cell
  • WAYNE293 LVPRO cell adapted to serum-free culture medium environment and application of WAYNE293 LVPRO cell
  • WAYNE293 LVPRO cell adapted to serum-free culture medium environment and application of WAYNE293 LVPRO cell

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] The screening process of WAYNE 293LVPRO cells of the present invention is as follows figure 1 As shown, the specific steps are as follows:

[0022] 1. Serum-free suspension acclimatization of adherent HEK293 cells (ECACC 85120602) in culture medium (QuaCell CD02 medium, purchased from Zhongshan Kangtian Shenghe Biotechnology Co., Ltd.), so that HEK293 cells can adapt to the suspension growth state.

[0023] The specific domestication method is as follows:

[0024] A. Cell recovery

[0025] Key equipment: shaker, biological safety cabinet

[0026] Cell recovery method: heat the culture medium in a 36.5-37.5°C water bath for 30-40 minutes. Take a cryopreservation tube of stable HEK293 cell population, quickly place it in a 36.5-37.5°C water bath, shake it slowly to make it completely melt; spray 75% ethanol on the surface of the cryopreservation tube, and then pass it into the organisms that have been irradiated with ultraviolet light (≥30min). In the safety cabinet, ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a WAYNE293LVPRO cell adapted to a serum-free culture medium environment and application of the WAYNE293LVPRO cell. The human embryonic kidney cell WAYNE 293 is preserved in China General Microbiological Culture Collection Center (CGMCC) on May 24, 2021, the address is Institute of Microbiology, Chinese Academy of Sciences, No.3, No.1 Yard, Beichen West Road, Chaoyang District, Beijing, the postal code is 100101, and the preservation number is CGMCC No.22348. The human embryonic kidney cell WAYNE 293 provided by the invention is derived from suspension culture of HEK293 cells in a serum-free culture medium without an anti-caking agent, has the characteristics of short doubling time, high growth density, high virus packaging efficiency and the like, and is an important support for industrial development in the field of cell and gene therapy.

Description

Technical field: [0001] The invention belongs to the field of cells, and in particular relates to a WAYNE293 LVPRO cell adapted to a serum-free culture medium environment, short doubling time, high cell growth density and high virus packaging efficiency and application thereof. Background technique: [0002] With the development of the cell and gene therapy industry, the method of using viral vectors to introduce target genes has become a new direction for human treatment of major diseases such as congenital genetic diseases, leukemia, and cancer. [0003] At present, the preparation of viral vectors is mainly carried out by using HEK293 as host cells and using chemical reagents such as cationic polymers for transient transfection. This is because HEK293 has certain advantages in transient transfection efficiency and virus packaging yield compared with other cells. However, most of the currently marketed gene and cell therapy drugs still use adherent culture, such as the me...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N5/073C12N5/071C12N5/02C12R1/91
CPCC12N5/0686C12N5/0603C12N2500/90
Inventor 喻晓瑜潘洪辉吴帆袁军胥文彦姜玉林彭慧张家欣
Owner QUACELL BIOTECHNOLOGY CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap