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Method for improving titer of T4 bacteriophage through cooperation of high-voltage electrostatic field and 1,3-diglyceride and collagen peptide

A collagen peptide, high-voltage electrostatic field technology, applied in the field of T4 phage, can solve problems such as unreported, and achieve the effects of simple operation, improved titer, and easy control

Pending Publication Date: 2021-11-26
QINGDAO AGRI UNIV
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

But at present, there is no report on the application of high-voltage electrostatic field to synergize 1,3-diglyceride and collagen peptides to increase the titer of T4 phage

Method used

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Examples

Experimental program
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Embodiment

[0023] A method for improving the titer of T4 bacteriophage in cooperation with 1,3-diglyceride and collagen peptide in a high-voltage electrostatic field, comprising the steps of:

[0024] S1. Pick a single colony from the preserved Escherichia coli AMC 198, put it into 5 mL LB liquid medium, shake it at 160 r / min at 37 °C for 8 h, and obtain 1.00×10 8 Escherichia coli AMC 198 bacterial suspension of cfu / mL concentration;

[0025] S2. Prepare 1.0% 1,3-diglyceride emulsion 1 mL, 0.5% collagen peptide aqueous solution 1 mL;

[0026] S3. The titer of freezing at -80°C is 5.00×10 8 The pfu / mL stock solution of T4 phage was thawed at 4°C. Take 100 μL of T4 phage stock solution, 100 μL of Escherichia coli AMC 198 bacterial suspension, inoculate in 5 mL LB liquid medium, add 200 μL of 1.0% 1,3-diglyceride emulsion and 200 μL of 0.5% collagen peptide aqueous solution , mixed evenly, and left to stand for 15 minutes to obtain a mixture of Escherichia coli AMC 198, T4 phage, 1,3-di...

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PUM

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Abstract

The invention discloses a method for improving the titer of a T4 bacteriophage through cooperation of a high-voltage electrostatic field and 1,3-diglyceride and collagen peptide. The titer of the T4 bacteriophage is improved by adding the 1,3-diglyceride and the collagen peptide and culturing the T4 bacteriophage under the high-voltage electrostatic field. The T4 bacteriophage cultured by the invention improves the bacteriophage effect on Escherichia coli AMC 198, is expected to replace antibiotics, has a wider application prospect in the field of bacteriophages, and has important significance in prevention and treatment of bacterial pollution in the fields of clinic, food and agriculture.

Description

technical field [0001] The invention relates to the field of bioengineering, in particular to a high-voltage electrostatic field cooperating with 1,3-diglyceride and collagen peptide to act on T4 phage to obtain a T4 phage with higher titer. Background technique [0002] Bacterial contamination has become a global problem that has undermined the social and economic stability of the environment, food, and healthcare systems. Colibacillosis is the cause of high morbidity, mortality and economic loss in the global livestock and poultry industry. Escherichia coli is considered an important zoonotic pathogen that causes foodborne urinary tract infections in humans from consumption of contaminated poultry (Goudarztalejerdi A, Mohammadzadeh A, Najafi S V, et al. Serogrouping, phylotyping, and virulence genotyping of commensal and avian pathogenic Escherichia coli isolated from broilers in Hamedan, Iran[J]. Comparative Immunology, Microbiology and Infectious Diseases, 2020, 73:1015...

Claims

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Application Information

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IPC IPC(8): C12N7/00C12N13/00
CPCC12N7/00C12N13/00C12N2795/10151Y02A50/30
Inventor 孙京新王宝维黄明王淑玲李岩郭丽萍张名爱蓝天婵黄河姚现琦张从祥赵向进郑远东郝小静
Owner QINGDAO AGRI UNIV
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