Method for creating disease-resistant high-quality common head cabbage germplasm resources

Abstract

The invention discloses a method for creating disease-resistant high-quality common head cabbage germplasm resources, and belongs to the technical field of cabbage breeding. The method comprises the following steps: respectively hybridizing a cabbage CMS sterile line / maintainer line with excellent comprehensive characters with cabbage fertile plants with excellent comprehensive characters, then respectively carrying out in-vitro tissue culture from filial generations, recombining and screening sterile plants with similar spheres, mature periods and colors and excellent comprehensive characters, and backcrossing the sterile plants with similar fertile plants for three generations; and after the fertile plants are subjected to selfing for one generation, carrying out inter-plant crossing, and cultivating new CMS sterile lines / maintainer lines with different maturity, spheres and colors and excellent comprehensive characters and other new excellent fertile plants. Through callus protoplast improved culture, the generation frequency of protoplast regenerated plants is improved, chimeras and polyploids are removed, excellent fertile plants are reserved, and more and better new germplasm resources are created.

Description

technical field [0001] The invention belongs to the technical field of cabbage breeding, and in particular relates to a method for creating germplasm resources of disease-resistant and high-quality head cabbage. Background technique [0002] Heading cabbage (Brassica oleacea L.var.capitata L.) is a variety of Brassica plants in the family Brassica, also known as cabbage, cabbage, etc. It originated from the Mediterranean coast and has a mild climate. It is the main vegetable crop in China. One of them was introduced into China in the 16th century. Heading cabbage (hereinafter referred to as cabbage) has the characteristics of disease resistance, strong adaptability, easy storage and transportation, high yield, and good quality. It is widely cultivated in various parts of our country and has developed rapidly. One of the main vegetables in summer, autumn and winter, with an annual planting area of ​​3 million hectares 2 All of the above plays an important role in the annual...

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Problems solved by technology

However, the use of self-incompatible lines for seed production requires a lot of manpower and time for parental breeding (through artificial pollination at the bud stage), which is very costly, and the promotion area is limited by the number of seeds produced.

What's more unfavorable is that the long-term continuous self-trading viability of the parents degrades, so that some excellent characters are lost, and the hybridization rate is difficult to reach 100%. However, when the cytoplasmic male sterile line (CMS male sterile line) is used to prepare excellent varieties of cabbage, the hybridization rate reaches 100%. %, do not need the restoration line, only the male parent line

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