Artificial cubilose and preparation method thereof
A bird's nest, artificial technology, applied in artificial cell constructs, food science, vertebrate cells, etc., can solve the problems of destroying the breeding of bird flocks, existing health risks, consumption of natural bird's nest ecological environment, etc., and achieve the effect of high nutritional value.
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preparation example Construction
[0059] A preparation method of artificial bird's nest, comprising:
[0060] The animal-derived gelatin mixed with epithelial cells is solidified into a gel-state culture layer, and the epithelial cells include salivary gland epithelial cells;
[0061] The collection layer of the ion-interactive macromolecular network structure is constructed by polysaccharides and edible gelatin, and the polysaccharides include positively charged polysaccharides and negatively charged polysaccharides;
[0062] The culture layer is placed over the collection layer, and the culture layer is submerged in the medium so that the collection layer collects cell secretions overflowing from the culture layer.
[0063] In the method provided in the examples of this application, in the culture layer, the salivary gland epithelial cells cultured in the gelatin matrix secrete various nutrients including sialic acid and growth factors, and these nutrients are charged, and the positively charged polysacchari...
Embodiment 1
[0096] 1. Preparation of culture layer
[0097] Fully dissolve 10g of pigskin-derived gelatin in 100mL of deionized water, place in a round bottom flask, heat to 50°C, and stir for 12 hours. Slowly add 8 mL of methacrylic anhydride dropwise into the gelatin solution, stir well, and place it at 50°C for 3 hours. After the reaction was finished, the reaction solution was taken out and dialysis was carried out for 7 days using a 12-14kDa dialysis bag, during which the water was changed several times and the operation was protected from light. The final solution is subjected to a freeze-drying operation to obtain a freeze-dried powder of photocurable gelatin.
[0098] Prepare 5mL photoinitiator standard solution, preheat to 60℃ for later use, weigh 0.25g photocurable gelatin freeze-dried powder, add the preheated initiator standard solution into the centrifuge tube containing photocurable gelatin, keep away from water bath at 60℃ Light dissolves for 30 minutes, during which time...
Embodiment 2
[0107] This example is basically the same as Example 1, except that the epithelial cells are human epithelial epithelial cells and mouse salivary gland epithelial cells, and the ratio of these two types of cells is 1:1~5 (such as 1:1, 1 :2, 1:3, 1:4 or 1:5), and the medium is RPMI-1640 medium.
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