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Artificial cubilose and preparation method thereof

A bird's nest, artificial technology, applied in artificial cell constructs, food science, vertebrate cells, etc., can solve the problems of destroying the breeding of bird flocks, existing health risks, consumption of natural bird's nest ecological environment, etc., and achieve the effect of high nutritional value.

Active Publication Date: 2021-11-30
UNIVERSITY OF MACAU
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, the acquisition of natural bird's nest has brought huge ecological environment consumption
First of all, edible bird's nests are made of swiftlets, and swiftlets are still unable to be cultured artificially. Javan swiftlets have soared from "least danger" to "extremely endangered" in the "China Biodiversity Red List"; Secondly, the natural bird’s nest generally takes 4 months to build, and some bird’s nest farms over-collect. In order to collect the bird’s nest as soon as possible, all the hatching mother swallows and eggs are dumped, which seriously damages the reproduction of the bird’s nest; Finally, in the process of forming the natural bird's nest, nitrite will be fermented and formed under the appropriate temperature and humidity, and at the same time, bird droppings containing a large amount of nitrate will also pollute the bird's nest, and even the tyrosine residues of the bird's nest itself and the activity in the environment The nitrogen reaction will also form blood swallows. Even the better natural bird's nests still contain nitrite, heavy metal ions, fungi and other substances harmful to human body, posing health risks

Method used

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  • Artificial cubilose and preparation method thereof
  • Artificial cubilose and preparation method thereof
  • Artificial cubilose and preparation method thereof

Examples

Experimental program
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Effect test

preparation example Construction

[0059] A preparation method of artificial bird's nest, comprising:

[0060] The animal-derived gelatin mixed with epithelial cells is solidified into a gel-state culture layer, and the epithelial cells include salivary gland epithelial cells;

[0061] The collection layer of the ion-interactive macromolecular network structure is constructed by polysaccharides and edible gelatin, and the polysaccharides include positively charged polysaccharides and negatively charged polysaccharides;

[0062] The culture layer is placed over the collection layer, and the culture layer is submerged in the medium so that the collection layer collects cell secretions overflowing from the culture layer.

[0063] In the method provided in the examples of this application, in the culture layer, the salivary gland epithelial cells cultured in the gelatin matrix secrete various nutrients including sialic acid and growth factors, and these nutrients are charged, and the positively charged polysacchari...

Embodiment 1

[0096] 1. Preparation of culture layer

[0097] Fully dissolve 10g of pigskin-derived gelatin in 100mL of deionized water, place in a round bottom flask, heat to 50°C, and stir for 12 hours. Slowly add 8 mL of methacrylic anhydride dropwise into the gelatin solution, stir well, and place it at 50°C for 3 hours. After the reaction was finished, the reaction solution was taken out and dialysis was carried out for 7 days using a 12-14kDa dialysis bag, during which the water was changed several times and the operation was protected from light. The final solution is subjected to a freeze-drying operation to obtain a freeze-dried powder of photocurable gelatin.

[0098] Prepare 5mL photoinitiator standard solution, preheat to 60℃ for later use, weigh 0.25g photocurable gelatin freeze-dried powder, add the preheated initiator standard solution into the centrifuge tube containing photocurable gelatin, keep away from water bath at 60℃ Light dissolves for 30 minutes, during which time...

Embodiment 2

[0107] This example is basically the same as Example 1, except that the epithelial cells are human epithelial epithelial cells and mouse salivary gland epithelial cells, and the ratio of these two types of cells is 1:1~5 (such as 1:1, 1 :2, 1:3, 1:4 or 1:5), and the medium is RPMI-1640 medium.

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Abstract

The invention relates to the cross technical field of food science, tissue engineering and biomaterial science, and discloses an artificial cubilose and a preparation method thereof. The preparation method of the artificial cubilose comprises the following steps of: solidifying animal-derived gelatin mixed with epithelial cells into a gel-state culture layer, wherein the epithelial cells comprise salivary gland epithelial cells; constructing a collection layer of an ion interaction macromolecular network structure by polysaccharides and edible gelatin, wherein the polysaccharides comprise positively charged polysaccharides and negatively charged polysaccharides; and placing the culture layer above the collection layer, and immersing the culture layer in a culture medium, so that the collection layer collects cell secretions overflowing from the culture layer. The artificial cubilose is prepared by adopting the above preparation method. The preparation in the invention does not relate to artificial breeding of swallows and does not relate to damage to wild swallows or other natural resources; the nutritional ingredients of the prepared artificial cubilose are not worse than those of natural cubilose; and furthermore, the prepared artificial cubilose almost does not contain substances such as nitrite, heavy metal ions and fungi which are harmful to human bodies.

Description

technical field [0001] The present invention relates to the interdisciplinary technical fields of food science, tissue engineering and biomaterials, in particular to artificial bird's nest and a preparation method thereof. Background technique [0002] Bird's nest is a nest made of saliva and down feathers secreted by swiftlets of the swift family (Apodidae) and various swallows of the same genus, and bird's nest products are bird's nests with feathers and other impurities removed. In traditional medicine, it is believed that it has the functions of nourishing lung yin, reducing phlegm and relieving cough, and regulating deficiency. It is regarded as a good nourishing product in China, Thailand, Singapore, Malaysia and other countries. In China alone, sales in 2020 will reach 50 billion yuan. Natural bird's nests are mainly produced in Southeast Asian countries such as Indonesia, Thailand, and Vietnam. According to the industry standard "Bird's Nest Quality Grade" for China...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L33/00A23L29/00A23L29/30C12N5/071
CPCA23L33/00A23L29/045A23L29/30C12N5/0625A23V2002/00A23V2200/30A23V2250/5024A23V2250/5432A23V2250/511
Inventor 王春明刘杨杨张哲廖祯诚
Owner UNIVERSITY OF MACAU
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