Lactobacillus fermentum and use thereof

A technology for fermenting lactobacillus and fermented rice, applied in the directions of lactobacillus, application, bacteria, etc., can solve the problems of unstable production quality, small processing scale and vague process of natural fermentation susceptible to bacterial infection, so as to improve cooking quality and improve Relative content, effect of reducing fermentation time

Active Publication Date: 2021-12-03
HENGYANG NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the technical level of the current rice noodle processing industry is still relatively backward. Most of them are produced in manual workshops, the processing scale is small, the process is vague, and the susceptibility to bacterial contamination of natural fermentation will also lead to unstable production quality.

Method used

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  • Lactobacillus fermentum and use thereof
  • Lactobacillus fermentum and use thereof
  • Lactobacillus fermentum and use thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0031] The present invention also provides a method of preparing a fresh-wet rice powder comprising the steps of: inoculation of the fermentation of Lactobacillus in the rice in the rice to obtain fermented rice; add the fermentation rice to the water, gelatin, squeeze, squeeze That is, fresh and wet rice noodles.

[0032] Starch is the main ingredient of rice, is divided into linear starch and branched starch, and rice can be used to prepare fresh wet rice noodles after fermentation. During the fermentation process, the propagation of bacteria produces an acid, degrades the protein that is wrapped in the starch, and the starch is released, thus accelerating the rate of starch aging and gelatinity; during the fermentation process, the brackets have broken and Dependent, the average degree of polymerization decreases, and since the degree of aggregation of the amylopeticles is inversely proportional to the gel properties of the rice noodles, the fermentation process can maintain th...

Embodiment 1

[0045] This embodiment provides a screening and identification process of fermentation Lactobacillus LFChen.

[0046] (1) Take 10ml of the rice fermentation liquid produced by Hunan Kang Jie Food Technology Development Co., Ltd., 10ml of rice flour, dilution 10 -1 10 -2 ...... 10 -7 A total of 7 gradients were placed in sequentially placed in a casting plate medium (MRS-calcium medium), and the culture time was 37 ° C, culture time 48 h, and transparent calcium occurred in the medium in the medium. The circle, and the single bacteria is rounded or elliptical, the color is white or milky white, labeled such a colony, initially determined as lactic acid bacteria.

[0047] (2) Take the single bacterial shipping line in step (1) to continue to cultivate, after purification is completed, see the results figure 1 (The mirror detection is 1000x). It can be seen that the obtained strain has no spores, and Gram is positive, and the shape is a long rod, a long average of about 1.29 μm, and ...

Embodiment 2

[0054] This example explores the effect of fermentation Lactobacillus LFCHEN on rice fermentation.

[0055] Fermentation Lactobacillus LFCHEN (RFB2-11) and the present invention while the other three different lactic acid bacteria (RFB2-3, RFB2-6, RFB2-10) screened from the rice fermentation liquid of Kang Jie rice noodles. . 500 ml of water is added to each 250g rice, and the final concentration is 10 7 CFU / ml of bacterial liquid fermentation at 37 ° C.

[0056] (1) When the fermentation time is 0 h, 12h, 18h, 24h, 36h, the fermentation rice of these 5 points points is compared with the pH of 0H, 24H and 48H fermented rice in the fresh moisture rice noodles in Kang Jie rice. See the results Image 6 . Depend on Image 6 It can be seen that the same fermentation time, the pH of the fermented Lactobacillus LFCHEN (RFB2-11) is up to the maximum pH of rice fermentation and tends to be stable, only 12 h, and the pH drop rate of other strains is slightly lower than the fermentation Lac...

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Abstract

The invention discloses a lactobacillus fermentum strain and use thereof, and belongs to the technical field of use of fermentation microorganisms. The strain is named as lactobacillus fermentum LF Chen and deposited in China Center for Type Culture Collection (CCTCC) on May 21, 2021 and has a deposit number of CCTCC NO: M 2021568. The provided lactobacillus strain can be used for fermenting rice, such that rice milk in a rice fermentation process can quickly reach the optimal fermentation pH, the production efficiency is improved, the relative content of amylose and total starch in the rice milk can be improved, and cooking quality of rice noodles is improved.

Description

Technical field [0001] The present invention relates to the field of fermentation microbial applications, and in particular, to a fermented Lactobacillus and its application thereof. Background technique [0002] The fresh wet rice flour is machined from the production process of rice as raw materials, soaking, grinding, gelatinating type, cooling, etc. Milin has started from China. It has been more than 200,000 years of history. After many years of development, there are many varieties of fresh and humid rice noodles in all parts of the country, Guilin rice noodles, Changde rice noodles and other national famous and deeply loved, rice noodles have become people in everyday life. Or lack of food. However, the current technical level of the rice powder is still relatively backward. It uses a lot of workshop production, small processing scale, blurred process, and natural fermentation can lead to unstable production quality. [0003] Studies on the fermentation process of fresh and...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23L7/104C12R1/225
CPCA23L7/104A23V2400/143
Inventor陈晓华陆慧肖婷李建周蒋莹魏薇邓悦
OwnerHENGYANG NORMAL UNIV