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Dry and hot valley region cauliflower tissue culture seedling hardening method

A dry-hot valley, tissue culture seedling technology, applied in horticultural methods, botanical equipment and methods, cultivation and other directions, can solve the problems of low survival rate of tissue culture seedlings, inability to accept strong light, seedling diseases, etc. The effect of hardening survival rate, reducing seedling diseases, and robust root system

Active Publication Date: 2021-12-21
RES INST OF TROPICAL ECO AGRI SCI YUNAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The hot and dry valley area has a hot and dry climate, with an average annual rainfall of 657.5 mm, an annual average temperature of 21.4°C, an extreme maximum temperature of 42°C, an annual average relative humidity of 53%, and about 7 months of dry season with less than 50 mm of precipitation; cauliflower tissue culture seedlings In the process of seedling hardening, it is very easy to cause seedling diseases due to high temperature. At the same time, in a dry and low-humidity environment, it is easy to cause leaf wilting. The survival rate of hardened seedlings is extremely low

Method used

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  • Dry and hot valley region cauliflower tissue culture seedling hardening method
  • Dry and hot valley region cauliflower tissue culture seedling hardening method
  • Dry and hot valley region cauliflower tissue culture seedling hardening method

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Embodiment 1

[0026] A method for improving the survival rate of cauliflower tissue culture seedling hardening and transplanting in dry-hot valley area, specifically carried out according to the following steps:

[0027] Step 1, putting sterile plants of different genotypes of cauliflower into rooting medium MS+0.1mg / LIBA+0.1mg / LIAA for rooting culture;

[0028] Step 2. When the root of the cauliflower tissue culture seedling grows to 1-1.5cm, move the tissue culture bottle containing the tissue culture seedling from the incubator to a cool indoor area (without direct sunlight) and place it in a sunny place after 2 days. The area of ​​the oblique shot is left for 3 days;

[0029] Step 3, Pinshi peat soil and vermiculite are mixed in a ratio of 2:1, and the mixed substrate is divided into 72-hole seedling trays; and the mixed solution of Liangdun and agricultural streptomycin is prepared for watering and set aside (Liangdun is 1500 times liquid, agricultural streptomycin is 3000 times liqui...

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Abstract

The invention discloses a dry and hot valley region cauliflower tissue culture seedling hardening method, and particularly relates to the technical field of tissue culture seedling hardening. The method comprises the following steps that a tissue culture seedling bottle is transferred from an incubator to an indoor shady and cool region, placed for 2 days, then transferred to a sunshine oblique irradiation region, and placed for 3 days; prepared matrixes are subpackaged into hole seedling trays; the tissue culture seedlings are taken out of the tissue culture seedling bottle, washed clean and soaked in a bactericide; single broccoli plants are correspondingly transplanted into the hole seedling trays, and irrigated with a rooting stimulating solution; a seedling tray is placed in a shading area, and the seedling tray is completely covered with a non-porous plastic cover with red and blue alternated on the first day; and the seedling tray is continuously covered with a red and blue plastic cover with a plurality of through holes from the second day to the seventh day, the cover is taken down after 7 days, alternate irrigating of water or fertilizer water is carried out during the period, and then the seedling tray is transplanted into a field. By means of the dry and hot valley region cauliflower tissue culture seedling hardening method, the seedling hardening survival rate of the cauliflower tissue culture seedlings in the dry and hot river valley region is greatly increased.

Description

technical field [0001] The invention relates to the technical field of cauliflower propagation, in particular to a method for cultivating cauliflower seedlings in dry-hot valley areas. Background technique [0002] Cauliflower is a variety of Brassica cabbage in the cruciferous family. It uses curds as edible organs. It is rich in nutrients, contains a lot of vitamins and glucosides, and has a crisp and tender taste, which is loved by consumers. It has become one of the most important vegetable crops in the world. The use of plant tissue culture can increase the reproductive coefficient of most highly self-incompatible lines of cauliflower and improve the breeding efficiency. [0003] The hot and dry valley area has a hot and dry climate, with an average annual rainfall of 657.5 mm, an annual average temperature of 21.4 °C, an extreme maximum temperature of 42 °C, an annual average relative humidity of 53%, and about 7 months of dry season with less than 50 mm of precipitat...

Claims

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Application Information

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IPC IPC(8): A01G22/00A01G7/06A01H4/00A01G13/02
CPCA01G22/00A01G7/06A01H4/002A01H4/008A01G13/0243Y02P60/21
Inventor 汪玲敏麻继仙但忠木万福杨龙李丽琼李易蓉吕维梧苏银玲唐正富陈光平杨子祥
Owner RES INST OF TROPICAL ECO AGRI SCI YUNAN ACAD OF AGRI SCI
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