Dry and hot valley region cauliflower tissue culture seedling hardening method
A dry-hot valley, tissue culture seedling technology, applied in horticultural methods, botanical equipment and methods, cultivation and other directions, can solve the problems of low survival rate of tissue culture seedlings, inability to accept strong light, seedling diseases, etc. The effect of hardening survival rate, reducing seedling diseases, and robust root system
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[0026] A method for improving the survival rate of cauliflower tissue culture seedling hardening and transplanting in dry-hot valley area, specifically carried out according to the following steps:
[0027] Step 1, putting sterile plants of different genotypes of cauliflower into rooting medium MS+0.1mg / LIBA+0.1mg / LIAA for rooting culture;
[0028] Step 2. When the root of the cauliflower tissue culture seedling grows to 1-1.5cm, move the tissue culture bottle containing the tissue culture seedling from the incubator to a cool indoor area (without direct sunlight) and place it in a sunny place after 2 days. The area of the oblique shot is left for 3 days;
[0029] Step 3, Pinshi peat soil and vermiculite are mixed in a ratio of 2:1, and the mixed substrate is divided into 72-hole seedling trays; and the mixed solution of Liangdun and agricultural streptomycin is prepared for watering and set aside (Liangdun is 1500 times liquid, agricultural streptomycin is 3000 times liqui...
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