Dry and hot valley region cauliflower tissue culture seedling hardening method
A dry-hot valley, tissue culture seedling technology, applied in horticultural methods, botanical equipment and methods, cultivation and other directions, can solve the problems of low survival rate of tissue culture seedlings, inability to accept strong light, seedling diseases, etc. The effect of hardening survival rate, reducing seedling diseases, and robust root system
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[0025] Example 1
[0026] A method of improving the transfusion of seedling seedlings in the cauliflower group in the dry heat river area, specifically in accordance with the following steps:
[0027] Step 1, add the sterile plants of different genotype cauliflower into the rooted medium MS + 0.1 mg / liBa + 0.1 mg / liaa for rooting;
[0028] Step 2, when the civi group is seedling to 1 to 1.5 cm, it will be placed in the indoor cool area (no direct sunlight) in the incubator. 3 days of oblique area;
[0029] Step 3, the brilliantla carbon soil is mixed with the proportion of 2: 1, and the mixed matrix is separated into the 72-well seedling tray; and the bright shield and the agronomycin mixed solution are spare (bright shield is 1500 times solution, agriculture of streptomycin is 3000 times liquid, after dilution, according to volume ratio 1: 1, after mixing);
[0030] Step 4, the group culture seedling is removed from the group culture bottle, rinse the root medium; and use b...
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