Animal testis extract and preparation method and application thereof

An extract and animal technology, applied in the direction of mammalian medical raw materials, drug combinations, diseases, etc., can solve the problems of testosterone side effects and safety, so as to increase testosterone content, promote growth and development, and gonad growth and development, which is very safe sexual effect

Pending Publication Date: 2021-12-24
北京第一生物化学药业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] Aiming at defects such as side effects and safety of synthetic testosterone in the prior art, the present invention provides an animal testis extract that can be safely and effectively used to treat oligospermia and asthenic infertility, and provides the animal testis extract The preparation method of extract and its application, thus completed the present invention

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  • Animal testis extract and preparation method and application thereof
  • Animal testis extract and preparation method and application thereof
  • Animal testis extract and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Embodiment 1: the preparation of testis extract

[0052] 1.1 Unfreeze selection

[0053] Thaw freshly frozen sheep testes and inspect their appearance, select good sheep testes, remove non-substantial parts such as fascia and fat, and weigh them. Wherein, the selection criteria are: select sheep testicles with neat appearance, no obvious damage and no corruption.

[0054] 1.2 Broken homogenate

[0055] Clean the selected testicles with purified water, crush them with a mixer until they are homogenized, weigh them after crushing, add 2 times the amount of purified water by weight, stir evenly, and put the homogenate in a freezer at -20°C~ Freeze at -40°C for more than 48 hours.

[0056] 1.3 melting

[0057] After freezing, it is thawed out of the warehouse, and the thawing temperature must not be higher than 18°C ​​to obtain the sheep testis freeze-thaw solution.

[0058] 1.4 Freeze drying

[0059] The sheep testis freeze-thaw liquid is introduced into the freeze d...

Embodiment 2

[0060] Embodiment 2 testis extract active ingredient identification

[0061] In this embodiment, the following methods are used to identify the active ingredients of the testis extract prepared in Example 1:

[0062] Western blotting (immunoblotting test) is Western Blot. It is an experimental method commonly used in molecular biology, biochemistry and immunogenetics. The basic principle is to stain the cells or biological tissue samples treated by gel electrophoresis through specific antibodies. Information about the expression of specific proteins in the analyzed cells or tissues can be obtained by analyzing the location and depth of staining.

[0063] Protein samples separated by PAGE (polyacrylamide gel electrophoresis) are transferred to a solid phase support (such as nitrocellulose membrane). The solid phase support adsorbs proteins in the form of non-covalent bonds, and can maintain the type and Its biological activity remains unchanged. Use the protein or polypepti...

Embodiment 3

[0086] Example 3: Testis extract promotes blood testosterone content increase and prostate safety experiment

[0087] 3.1 Experimental materials

[0088] BALB / C mice (6-8 weeks), male (non-castrated), purchased from the Animal Department of Peking University Health Science Center;

[0089]Testis extract suspension preparation: the testis extract prepared according to the method described in Example 1 was diluted with an appropriate volume of ultrapure water according to the requirements in the experimental method described in section 3.2 below to make a suspension; testosterone Suspension preparation: testosterone (Annaiji company, 5g pack, purity 98%) was dissolved in an appropriate volume of ultrapure water to make a suspension; testosterone ELISA detection kit: purchased by RnD company; multi-band microplate reader (MULTISKAN MK3, American Thermo Company); some dissecting scissors, tweezers; conventional balance.

[0090] 3.2 Experimental method

[0091] 40 mice were div...

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Abstract

The invention discloses an animal testis extract and a preparation method and application thereof. The invention provides a preparation method of an animal testis extract, which is characterized by comprising the following steps: (1) inspecting the appearance of an animal testis, and removing a non-substantial part; (2) crushing and homogenizing the animal testis; (3) freezing and unfreezing the homogenized animal testis to obtain animal testis freeze-thawed liquid; and (4) freeze-drying the animal testis freeze-thawed liquid. Compared with artificially synthesized testosterone, the animal testis extract provided by the invention has better biological safety. The animal testis extract provided by the invention also has the functions and effects of promoting sexual desire of human or male animals, promoting growth and development of human or male animals and gonad growth and development, and increasing and recovering the sperm quantity of human or male animals.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to an animal testis extract and its preparation method and application. Background technique [0002] Infertility has become a major problem in the development of today's society. According to the report of the authoritative magazine "The Lancet" in 2020, about 1 / 5 couples in the world are affected by infertility, about 50% of the couples are male factors are the main reason, and the vast majority of male infertility Infertility is due to sperm problems. With the development of modern society, the delay of childbearing age has further increased the prevalence of infertility. The sperm count of modern men continues to decline significantly. With the increase of age, the decline of testosterone content is an irreversible trend. Testosterone levels are closely related to testicular reserve, fertility, and sperm quantity and quality. [0003] Infertility is an important issue that affec...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/52A61P15/08A61P15/00A61P15/10
CPCA61K35/52A61P15/08A61P15/00A61P15/10
Inventor 张博文康志云郑新越王烈
Owner 北京第一生物化学药业有限公司
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