SSR primer pair of Nymphaea tetragona, and synthesis method and application of SSR primer pair

Abstract

The invention discloses an SSR primer pair of Nymphaea tetragona, and a synthesis method and application of the SSR primer pair. The synthesis method comprises the following steps of: taking a Nymphaea colorata Peter whole genome as a database, retrieving SSR information sites, synthesizing an initial SSR primer pair, carrying out fluorescence labeling PCR amplification, then, carrying out fluorescence capillary electrophoresis detection, and carrying out band typing analysis to obtain a final SSR primer pair. The primer pair is good in stability, high in polymorphism and high in specificity, and can be used for constructing the phylogenetic trees and the DNA fingerprint spectrums of the Nymphaea tetragona.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to a pair of SSR primers for water lilies, a synthesis method and an application. Background technique [0002] Water lily is the collective name of plants of the genus Nymphaea L. in the family Nymphaeaceae. It is a perennial aquatic perennial flower and a famous flower in the world. According to ecological characteristics, it can be divided into cold-resistant water lilies and tropical water lilies. Water lilies have beautiful flowers and leaves and strong aroma. Fragrant, the flowers can be made into tea, and the petioles and flower stalks are edible. Some tropical water lilies have thick and straight stems, which rise above the water surface. [0003] Germplasm resources are the material basis for plant genetic improvement. Water lily germplasm resources are the material basis for the development of water lily fresh cut flowers and edible production. And artificial selection, there...

AI Technical Summary

Problems solved by technology

[0003] Germplasm resources are the material basis for plant genetic improvement. Water lily germplasm resources are the material basis for the development of water lily fresh cut flowers and edible production. And artificial selection, there are a large number of water lily species resources on the market, but the genetic background of different varieties is unknown, and the phenomenon of homonyms and different names and homonyms is more common, resulting in an increase in the redundancy of germplasm resources. In addition, under the trend of continuous updating of new varieties, some traditional representative varieties have gradually withdrawn from the market due to low prices, resulting in loss of resources. It is of great significance to select the minimum number of germplasm resources to represent the genetic diversity of germplasm resources to the greatest extent, and to protect and utilize them

[0004] At present, core collections are mainly constructed based on two types of data, morphological markers and molecular markers. For aquatic flowers such as water lilies, it is difficult to collect and preserve resources, and phenotypes are easily affected by seasonal and environmental changes, but this impact is inevitable. Inherited, the genetic material at the gene level has not changed, so morphological markers are not advisable. The use of molecular markers can more accurately identify and classify a variety of flowers, etc. However, the method of building core germplasm has a large workload. Operators will be exposed to toxic and harmful chemicals, such as mercaptoethanol, silver nitrate, etc., which will endanger their health, and the constructed primers are not high in specificity, and are easily affected by the environment for species identification.

Images