Embryo vitrification method and freezing liquid used
A technology of vitrification and freezing liquid, which is applied in the field of bovine in vitro fertilization, vitrification of bovine in vitro fertilized embryos, straws, embryo freezing tubes, vitrification of bovine in vitro fertilized embryos, and can solve the problems of inconvenient use
Active Publication Date: 2022-06-03
天津力牧生物科技有限公司
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Problems solved by technology
For example, CN203040526U (application number 2012202190736) discloses a thin straw used for vitrification of embryos, but this thin straw is very inconvenient to use for the embryo freezing tube operation that requires precise handling
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The invention relates to an embryo vitrification freezing method and a freezing liquid used. Specifically, the method for culturing bovine in vitro fertilized embryos provided includes the following steps: providing vitrification liquid, freezing pretreatment liquid, thawing liquid, and freezing tubes; Inhale into the tube: thawing liquid, air, vitrification liquid, air, vitrification liquid containing embryos, air, thawing liquid; respectively seal the upper part of the thawing liquid at the upper end and the lower part of the lower end of the thawing liquid, so that the seven sections of suction are sealed In a cryovial, then freeze the cryovial in liquid nitrogen. Wherein the freezing tube comprises: a tube body; an expansion part, the lower part of which communicates with the upper end of the tube body; an information label arranged above the expansion part; and a nozzle arranged at the lower end of the tube body. The method of the present invention and the used freezing liquid exhibit excellent effects as described in the specification.
Description
Embryo vitrification methods and freezing solutions used technical field The invention belongs to the technical field of animal reproduction, relate to a kind of technology for agriculture-animal husbandry veterinary reproduction, specifically relate to And a method for bovine in vitro fertilization, in addition, the present invention also relates to the relevant working test solution used in bovine in vitro fertilization in bovine body The application in in vitro fertilization, in particular, relates to a vitrification method for bovine in vitro fertilization embryos with excellent effect. this hair The vitrification method of Ming cattle in vitro fertilization embryo has excellent technical effect. The application also additionally relates to an embryo freezing Tubes, such as straws that can be used for vitrification of bovine IVF embryos. The embryo vitrification method of the present application can be By using the embryo freezing tube described in this applicat...
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IPC IPC(8): A01N1/02
CPCA01N1/021A01N1/0221A01N1/0226A01N1/0268
Inventor 王娜赵明礼郝少强鲁晶晶郭春明
Owner 天津力牧生物科技有限公司