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Bacillus cereus X-32 with high yield of C21 steroid compound and application of bacillus cereus X-32

A technology of Bacillus cereus and steroidal compounds, which is applied in the field of biopharmaceuticals, can solve the problems of complex extraction process, long production cycle, and large solvent consumption, and achieve the effect of less synthesis steps, short production cycle and high conversion rate

Pending Publication Date: 2022-01-04
YANCHENG TEACHERS UNIV
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Problems solved by technology

Preparation of C using plant extraction route 21 Steroidal compounds have disadvantages such as complex extraction process, long production cycle, large solvent consumption and difficult recovery

Method used

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  • Bacillus cereus X-32 with high yield of C21 steroid compound and application of bacillus cereus X-32
  • Bacillus cereus X-32 with high yield of C21 steroid compound and application of bacillus cereus X-32
  • Bacillus cereus X-32 with high yield of C21 steroid compound and application of bacillus cereus X-32

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Embodiment Construction

[0026] In order to make the object, technical solution and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0027] 1. Isolation, purification and screening of endophytic bacteria

[0028] 1. Sample pretreatment

[0029] The samples of Baishouwu collected from the planting base of Guolaoshouwu in Binhai County, Jiangsu Province were pretreated.

[0030] Put the fresh Radix Polygoni Multiflori that has been washed with tap water into an ultra-clean workbench, sterilize it with ultraviolet light for 10 minutes, rinse it with sterile water for 3 times, absorb the surface moisture with absorbent paper, and soak the roots, stems, leaves and seeds with 75% ethanol solution for 5 minutes , rinsed with sterile ...

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Abstract

The invention discloses bacillus cereus X-32 for high yield of a C21 steroid compound and application of the bacillus cereus X-32. The bacillus cereus X-32 is subjected to chemical character analysis and molecular identification to be bacillus cereus and is preserved in the China Center for Type Culture Collection in September 13, 2021, the preservation number is CCTCC NO: M 20211165, the preservation address is Wuhan University preservation center, Luoma, Wuchang District, Wuhan City, and the postcode is 430072. After the bacillus cereus X-32 is subjected to optimized fermentation culture, the concentration of C21 steroid in fermentation liquor reaches 1.280 + / -0.005 mg / mL, and a metabolite of the bacillus cereus X-32 is identified as cynanchum bungeanum glycoside C1N. The bacillus cereus X-32 is a new way for synthesizing the C21 steroid compound by microorganisms, and has the advantages of few synthesis steps, short production period, high conversion rate, easiness in regulation and control of the fermentation process, environmental friendliness and the like.

Description

technical field [0001] The invention belongs to the field of biopharmaceuticals, in particular to a high-yield C 21 Steroidal compounds of Bacillus cereus X-32 and their applications. Background technique [0002] C 21 Steroids are widely used in various fields, and have significant biological activities in anti-tumor, immune regulation, anti-oxidation, liver protection, blood lipid reduction, chemotherapy drug synergism and toxicity reduction, and anti-depression. C 21 Steroidal compounds mainly exist in plants such as radish, Tongguanteng, Baishouwu, Qingyangshen and black vine. Preparation of C using plant extraction route 21 Steroidal compounds have disadvantages such as complex extraction process, long production cycle, large solvent consumption and difficult recovery. [0003] Relevant studies have shown that plant endophytes and plants have the same secondary metabolite synthesis pathway, which can produce the same or similar medicinal active ingredients as the h...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P33/20A61K35/742A61P35/00C12R1/085
CPCC12P33/20A61K35/742A61P35/00Y02A50/30
Inventor 王欢莉夏文龙范忠军滕建蓉王成
Owner YANCHENG TEACHERS UNIV
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