Application of glycerol trinitrate and disodium fumarate in preparation of rumen methane inhibitor
A technology of disodium maric acid and glycerin trinitrate, which is applied in the fields of application, additional food elements, animal feed, etc., can solve the problems of unstable rumen methane effect and influence on rumen fermentation, and achieve the purpose of inhibiting rumen methane production and improving The effect of hydrogen accumulation and improvement of abnormal rumen fermentation
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[0012] Example 1. Triglycerol trinitrate and fumaric acid disodium salt combined preparation regulates rumen fermentation in vitro and reduces methanogenesis test.
[0013] 1 Material method
[0014] 1.1 Experimental materials
[0015] 1.1.1 Experimental animals and collection of rumen fluid
[0016] The experimental animals were 6 Hu sheep with permanent rumen fistula. Feed alfalfa hay and drink water ad libitum. Feed twice a day at 08:00 and 16:00. On the day of the test, rumen juice was taken half an hour before morning feeding, and fresh rumen juice was obtained from 6 fistula sheep and quickly filled into a thermos bottle filled with carbon dioxide and brought back to the laboratory.
[0017] 1.1.2 Preparation of artificial rumen fermentation broth
[0018] The preparation of fermented broth refers to the method of Menke and Steingass (1988). Filter the collected fresh rumen fluid with 4 layers of gauze, measure 1250ml of rumen fluid and quickly pour it into the pre...
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