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A Helicobacter pylori cd4 + T cell tolerance polypeptide fusion antigen and its application

A technology of Helicobacter pylori and fusion antigen, applied in the direction of bacterial antigen components, applications, peptides, etc., can solve the problems of ineffective stimulation of B cell immune response, Helicobacter pylori is not easy to clear, etc., to prevent and treat Helicobacter pylori infection , Improve the effect of clearing ability

Active Publication Date: 2022-07-15
FOURTH HOSPITAL OF HEBEI MEDICAL UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] Thus, OMV content can mediate progeny CD4 + T cells undergo selective apoptosis and cannot effectively stimulate B cells and CD8 + The immune response of T cells and macrophages, which leads to the innate resistance of the offspring to Helicobacter pylori and is not easy to clear

Method used

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  • A Helicobacter pylori cd4 <sup>+</sup> T cell tolerance polypeptide fusion antigen and its application
  • A Helicobacter pylori cd4 <sup>+</sup> T cell tolerance polypeptide fusion antigen and its application
  • A Helicobacter pylori cd4 <sup>+</sup> T cell tolerance polypeptide fusion antigen and its application

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Embodiment Construction

[0026] The technical solutions in the embodiments of the present invention will be described clearly and completely below. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0027] (one) Hp the cultivation of

[0028] will SS1 Hp Planted in dextrin-containing brain infusion broth (5mg / L trimethoprim, 5mg / L polymixin-B, 5mg / L amphoteracin-B, 10mg / L vancomucin) in a three-gas incubator (10% CO 2 , 85% N 2 , 5% O 2 ) at 37°C, 120 rpm, shaking culture for 3 to 10 days.

[0029] (2) Rough extraction Hp OMV

[0030] will be cultured to the logarithmic growth phase Hp The bacterial liquid was centrifuged at 10,000 × g for 10 min, the supernatant was collected, filtered with a 0.22 μm...

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Abstract

The invention discloses a Helicobacter pylori CD4 + The preparation process of T cell tolerance polypeptide fusion antigen and the polyclonal antibody milk prepared by using the antigen can be used for the treatment of infected patients with natural immune tolerance of Helicobacter pylori, with no drug resistance, good patient compliance and easy The advantages of large-scale production and so on.

Description

technical field [0001] The present invention relates to a kind of Helicobacter pylori CD4 + T cell tolerance polypeptide fusion antigen and its prepared polyclonal antibody milk. Background technique [0002] Helicobacter pylori ( Helicobacter pylori, Hp ) is a Gram-negative bacterium that accompanies human evolution. Currently for Hp Treatment is primarily a combination of two or three antibiotics with a proton pump. Rising antimicrobial resistance, recurrent infections, and high treatment costs limit the need for Hp Treatment. Prevention and treatment Hp Infection still lacks ideal countermeasures. [0003] Helicobacter pylori infection can cause systemic and localized gastric mucosal immune responses. Usually, neutrophils are recruited into the site of Helicobacter pylori infection in the gastric mucosa. If the neutrophils cannot clear Helicobacter pylori in time, the dendritic cells in the gastric mucosa will be activated, and then B cells will be recruited to...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/205C12N15/31A61K39/02A61P31/04
CPCC07K14/205C07K16/121A61K39/105A61P31/04
Inventor 单保恩赵连梅史中立胡代伦李晓亚
Owner FOURTH HOSPITAL OF HEBEI MEDICAL UNIV