Novel coronavirus antibody colloidal gold immunochromatography detection card and application thereof

A coronavirus and immunochromatography technology, applied in immunoassays, measuring devices, and analytical materials, can solve the problems of cumbersome detection steps and time-consuming, and achieve fast and accurate detection methods, simple and fast sampling, and less crossover effects

Pending Publication Date: 2022-03-01
HUAZHONG UNIV OF SCI & TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to solve the problems that the existing new coronavirus antibody detection technology has cumbersome and time-consuming detection steps, requires professionals to operate and mainly relies on laboratories, the present invention provides a new coronavirus antibody colloidal gold immunochromatography detection card and Detection method

Method used

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  • Novel coronavirus antibody colloidal gold immunochromatography detection card and application thereof
  • Novel coronavirus antibody colloidal gold immunochromatography detection card and application thereof
  • Novel coronavirus antibody colloidal gold immunochromatography detection card and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Embodiment 1: the preparation of detection card component

[0048] 1. Preparation of gold standard substances

[0049] 1.1 Preparation of colloidal gold solution

[0050] The colloidal gold solution was prepared by trisodium citrate reduction method, the colloidal gold particle size was basically the same, the distribution was even, and the diameter of the colloidal gold particle was about 20-60nm.

[0051] 1.2 Preparation of colloidal gold-coupled markers

[0052] 1.2.1 Preparation of conjugated markers of novel coronavirus recombinant N protein and colloidal gold

[0053] Use 0.2mol / L potassium carbonate solution to adjust the pH value of the colloidal gold solution to 9.0, gradually dilute the purified novel coronavirus recombinant N protein and mix with the colloidal gold solution, then add 10% sodium chloride solution and mix well , standing at room temperature for 2 hours to observe the results, the amount of the new coronavirus recombinant N protein added is n...

Embodiment 2

[0065] Example 2: New coronavirus IgM+IgG antibody detection method

[0066] (1) Take out the test card from the plastic package and place it on a dry table. The test card shows the quality control area (C) and the test area (G, M);

[0067] (2) Take fingertip blood with a blood collection device, stop the bleeding after blood collection, and add the blood sample to the sampling hole;

[0068] (3) Unscrew the cap of the diluent bottle, and drop 2 drops of diluent into the sample hole where the blood sample was dropped; read the result within 15 minutes, and do not read the result after 20 minutes.

[0069] (4) Interpretation of results:

[0070] Positive: IgG positive, a purple band appears in the test area (G), and a purple band appears in the quality control area (C); IgM positive, a purple band appears in the test area (M), A purple-red band appears in the quality control area (C); IgG positive and IgM positive, two purple-red bands appear in the test area (G, M), and a p...

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Abstract

The invention relates to a novel coronavirus antibody colloidal gold immunochromatography detection card and application, and belongs to the technical field of virus colloidal gold immunochromatography detection cards. The detection card comprises a bottom plate, a sample pad, a gold-labeled combination pad, a blood filter membrane, a nitrocellulose membrane and an absorption pad, the sample pad, the gold-labeled combination pad, the blood filtering membrane, the nitrocellulose membrane and the absorption pad are sequentially adhered to the bottom plate from left to right, the sample pad is connected with the gold-labeled combination pad, the gold-labeled combination pad is connected with the blood filtering membrane, the blood filtering membrane is connected with the nitrocellulose membrane, and the nitrocellulose membrane is connected with the absorption pad; a detection line G line, a detection line M line and a quality control line are distributed on the nitrocellulose membrane; according to the detection card, a capture method is adopted, novel coronavirus IgM and IgG antibodies in human whole blood are detected through an IgG secondary antibody and an IgM secondary antibody which are coated on a nitrocellulose membrane and a novel coronavirus antigen which is coated on a gold-labeled combination pad by utilizing a colloidal gold immunochromatography principle, and the detection method is rapid and accurate.

Description

technical field [0001] The present invention relates to the technical field of virus colloidal gold immunochromatography detection card, in particular, to a novel coronavirus antibody colloidal gold immunochromatography detection card and its application. Background technique [0002] The International Committee on Taxonomy of Viruses (ICTV) announced on February 11, 2020 that the official classification of the new coronavirus (2019-nCoV) was named severe acute respiratory syndrome coronavirus 2 (severe acute respiratory syndrome coronavirus 2). 2, SARS-CoV-2). The World Health Organization (WHO) announced on the same day that the official name of the disease caused by the virus is COVID-19. Coronaviruses are a large family of viruses known to cause colds as well as more serious illnesses such as Middle East Respiratory Syndrome (MERS) and Severe Acute Respiratory Syndrome (SARS). The novel coronavirus is a new strain of coronavirus that has never been found in humans befo...

Claims

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Application Information

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IPC IPC(8): G01N33/569G01N33/558G01N33/543
CPCG01N33/56983G01N33/558G01N33/54346G01N2469/20G01N2333/165
Inventor 杨海杨祥良张德军郭文胜杨宝凤金伟张婉容
Owner HUAZHONG UNIV OF SCI & TECH
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