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Thin-layer chromatography identification method for rhus chinensis

A technology of thin-layer chromatography and chrysanthemum, which is applied in the field of identification of Chinese medicinal materials, can solve the problems of few reports on identification methods of chrysanthemum, and achieve the effects of safe and reliable medication, fast development speed, and good separation effect

Pending Publication Date: 2022-03-04
广西壮族自治区食品药品检验所
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Problems solved by technology

At present, there have been some relevant reports on the chemical composition and content determination of Saltwood, but there are few reports on the identification method of Saltwood

Method used

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  • Thin-layer chromatography identification method for rhus chinensis
  • Thin-layer chromatography identification method for rhus chinensis
  • Thin-layer chromatography identification method for rhus chinensis

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Embodiment 1

[0032] A kind of thin-layer chromatography identification method of P. chinensis, described identification method comprises the following steps:

[0033] (1) Preparation of the test solution and the reference medicinal material solution: Take 1g of sarcocarpus powder, add 25ml of ethanol with a volume concentration of 60%, ultrasonically treat for 30 minutes, filter, evaporate the filtrate to dryness, add 2ml of methanol to the residue to dissolve, As the test solution; in addition, take 1 g of the reference drug powder of Salmonella chinensis, and make the reference drug solution in the same way;

[0034] (2) Preparation of reference substance solution: take myricetin reference substance and add ethanol with a volume concentration of 60% to make a solution containing 0.2mg per 1ml as the reference substance solution;

[0035] (3) According to the test of thin-layer chromatography (Chinese Pharmacopoeia 2015 Edition General Rule 0502), draw 2 μL each of the test solution, the ...

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Abstract

The invention relates to the technical field of identification of traditional Chinese medicinal materials, in particular to a thin-layer chromatography identification method of rhus chinensis. According to the thin-layer chromatography identification method, myricetin is taken as a reference substance, and a rhus chinensis sample is identified by matching with a rhus chinensis reference medicinal material. The rhus chinensis is analyzed by adopting the method, and the obtained thin-layer chromatography is good in separation effect, round and clear in spot, moderate in specific shift value and good in reproducibility; the method has the advantages of simple operation, simple equipment, easy color development, fast development rate and easy result determination, provides a basis for rhus chinensis identification, and provides reliable guarantee for medication safety of rhus chinensis.

Description

technical field [0001] The invention relates to the technical field of identification of traditional Chinese medicinal materials, in particular to a thin-layer chromatography identification method of chrysanthemum japonicus. Background technique [0002] Saltwood Rhus chinensis Mill. dry whole plant. Harvested in summer and autumn, washed, and dried; aliases: Yanshuang Cypress, Hydrochloric Acid Wood, Fuyan Tree, Pulian Salt, Laodan Salt, Gallnut Tree. Sour skin acid, slightly bitter, cool in nature; it has the effects of relieving cough, stopping bleeding, astringent, detoxifying, etc.; it is mainly used for phlegm cough, bloody stool, bloody dysentery, night sweat, carbuncle, ulcer, eczema, and snake bites. In my country except Northeast China, Inner Mongolia and Xinjiang, it is found in other provinces and autonomous regions. It grows in sunny hillsides, valleys, and streamside forests or thickets at an altitude of 170-2700 meters; distributed in India, Indochina Penins...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/90
CPCG01N30/90
Inventor 谢培德黄清泉李丽莉滕爱君林敬开罗永强
Owner 广西壮族自治区食品药品检验所
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