Medicine for resisting novel coronavirus SARS-CoV-2 and application thereof

A coronavirus, sars-cov-2 technology, used in antiviral agents, drug combinations, medical preparations containing active ingredients, etc., can solve the problems of patient injury, toxic side effects, etc. , the effect of inhibiting infection

Active Publication Date: 2022-03-18
INST OF PATHOGEN BIOLOGY CHINESE ACADEMY OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the above drugs have toxic side effects and may cause unnecessary damage to the patient itself, so finding a less toxic anti-SARS-CoV-2 drug has become an urgently needed solution for the treatment of COVID-19

Method used

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  • Medicine for resisting novel coronavirus SARS-CoV-2 and application thereof
  • Medicine for resisting novel coronavirus SARS-CoV-2 and application thereof
  • Medicine for resisting novel coronavirus SARS-CoV-2 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0024] African green monkey kidney cell Vero was purchased from ATCC; SARS-CoV-2 virus was isolated from the sample; one-step absolute quantitative PCR enzyme Fast Virus 1-Step Master Mix (4444434), Trizol LS was purchased from Thermo; nucleic acid extraction kit Direct- zol TMRNA MiniPrep (R2052) was purchased from Zymo Research; CCK8 kit (40203) was purchased from Shanghai Yisheng Biotechnology Co., Ltd.

[0025] 1. RNA extraction from the supernatant

[0026] Follow the instructions of the nucleic acid extraction kit, as follows:

[0027] 1) Add an equal volume of absolute ethanol to the sample added with Trizol LS, mix well and transfer the liquid to Zymo-Spin TM On the IICR column, the column is placed on the collection tube and centrifuged at 10000g for 1min

[0028] 2) Put the Zymo-Spin TM IICR column transferred to a new collection tube

[0029] 3) On Zymo-Spin TM Add 400 μl of Direct-zol to IICR column TM RNA PreWash, centrifuge at 10000g for 1min, discard ...

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PUM

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Abstract

The invention discloses a series of anti-novel coronavirus SARS-CoV-2 drugs and application thereof, in vitro cell test results show that the compound can significantly inhibit the infection of SARS-CoV-2 on normal cells, has an inhibition effect on novel coronavirus SARS-CoV-2 at the cellular level, can significantly reduce the virus titer of the virus in the cells, and can be used for preparing anti-novel coronavirus SARS-CoV-2 drugs. The proliferation of the compound in cells is inhibited, and the compound has dose dependence. Therefore, the compound can be used as a novel coronavirus SARS-CoV-2 inhibitor, and has the potential of treating COVID-19 pneumonia caused by human infection or other diseases caused by animal infection.

Description

technical field [0001] The present invention belongs to the field of virology and medicinal chemistry, and in particular relates to a compound that inhibits the replication of the novel coronavirus SARS-CoV-2 virus, the mechanism by which the compound inhibits the replication of the SARS-CoV-2 virus, and the use of the compound in preventing and treating SARS-CoV-2 -Related diseases caused by CoV-2 virus, especially the use on diseases related to inflammation and autoimmunity. Background technique [0002] The novel coronavirus SARS-CoV-2, the Middle East respiratory syndrome coronavirus MERS-CoV, and the severe acute respiratory syndrome coronavirus SARS-CoV belong to the family Coronaviridae. Strand RNA virus, whose genome length is about 26000-32000bp, is the largest RNA virus known so far. [0003] There is an urgent need for effective antiviral drugs to combat the infection of SARS-CoV-2, which can not only reduce the disease burden of patients, but also reduce the ris...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K45/00A61K45/06A61P31/14A61P11/00
CPCA61K45/06A61P31/14A61K45/00A61P11/00
Inventor 王健伟雷晓波肖霞任丽丽王聪慧
Owner INST OF PATHOGEN BIOLOGY CHINESE ACADEMY OF MEDICAL SCI
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