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A method for one-step nucleic acid detection using a liquid crystal sensor

A sensor and nucleic acid sensor technology, applied in the field of sensors, can solve the problems of reducing the detection efficiency of liquid crystal nucleic acid sensors, changing the optical image of the sensor, affecting stability, etc., and achieve the effects of fast detection speed, strong universality, and good stability

Active Publication Date: 2022-05-27
ZHEJIANG LAB
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In this three-step method, the competition between electrostatic interaction and hydrogen bond interaction takes a long time. On the one hand, the detection efficiency of the liquid crystal nucleic acid sensor is reduced. On the other hand, the optical image of the sensor is changed due to liquid phase volatilization, which affects its stability. sex

Method used

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  • A method for one-step nucleic acid detection using a liquid crystal sensor
  • A method for one-step nucleic acid detection using a liquid crystal sensor
  • A method for one-step nucleic acid detection using a liquid crystal sensor

Examples

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Effect test

Embodiment 1

[0053] Embodiment 1, utilize liquid crystal sensor to realize one-step detection of SARS virus oligonucleotide

[0054] In this embodiment, the micro-grid mesh film used for preparing the liquid crystal sensor is a micro-grid nickel mesh, and the liquid crystal used is E7 liquid crystal. After pouring the E7 liquid crystal, the sensor was left at 90°C for 20 min to ensure that the E7 liquid crystal completely entered the grid of the microgrid nickel mesh. In the detection process, the surfactant used is cetyl trimethyl ammonium bromide (CTAB), and the nucleic acid aptamer is the complementary DNA single strand of SARS virus oligonucleotide, and its sequence is 5'-GCA TCA CCG GAT GAT-3', the nucleic acid to be tested is SARS virus oligonucleotide, and its sequence is 5'-AUC AUC CGG UGA UGC-3'. like figure 1 As shown, if the existing "three-step method" is used for detection, the detection process is complicated, the detection time is long, and the optical image is unstable. ...

Embodiment 2

[0058] Example 2. One-step detection of novel coronavirus oligonucleotides using liquid crystal sensors

[0059] In this embodiment, the microgrid film used for preparing the liquid crystal sensor is a microgrid copper mesh, and the liquid crystal used is E7 liquid crystal. After pouring the E7 liquid crystal, the sensor was left at 80 °C for 30 min to ensure that the E7 liquid crystal completely entered the microgrid. During the detection process, the surfactant used is dodecyl trimethyl ammonium bromide (DTAB), and the nucleic acid aptamer is the complementary DNA single strand of the new coronavirus oligonucleotide, and its sequence is 5'-GCA TCT CCT GAT GAG-3', the nucleic acid to be tested is a new coronavirus oligonucleotide, and its sequence is 5'-CUC AUC AGG AGA UGC-3'. If the existing "three-step method" is used for detection, the detection process is complicated, the detection time is long, and the optical image is unstable. Therefore, this embodiment uses the "one...

Embodiment 3

[0063] Example 3. One-step detection of novel coronavirus oligonucleotides using liquid crystal sensors

[0064] In this embodiment, the microgrid film used for preparing the liquid crystal sensor is a microgrid copper mesh, and the liquid crystal used is E7 liquid crystal. After pouring the E7 liquid crystal, the sensor was left at 80 °C for 30 min to ensure that the E7 liquid crystal completely entered the microgrid. During the detection process, the surfactant used is dodecyl trimethyl ammonium bromide (DTAB), and the nucleic acid aptamer is the complementary DNA single strand of the new coronavirus oligonucleotide, and its sequence is 5'-GCA TCT CCT GAT GAG-3', the nucleic acid to be tested is a new coronavirus oligonucleotide, and its sequence is 5'-CUC AUC AGG AGA UGC-3'. If the existing "three-step method" is used for detection, the detection process is complicated, the detection time is long, and the optical image is unstable. Therefore, this embodiment uses the "one...

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Abstract

The present invention proposes that the present application discloses a method for one-step nucleic acid detection using a liquid crystal sensor, comprising the following steps: S1, preparing several liquid crystal nucleic acid sensors; S2, determining the specific volume fraction of the surfactant solution required for detection; S3, add the nucleic acid solution to be tested to the nucleic acid aptamer solution, and add the surfactant solution of the specific volume fraction obtained in step S2 to obtain the test solution; S4, add the test solution to the liquid crystal nucleic acid sensor, and observe Optical images to record the detection results. The invention can solve the problems of long detection time and unstable optical image faced in the current "three-step method" detection process, can realize instantaneous detection, and has the advantages of fast detection speed, good stability, high sensitivity and strong universality .

Description

technical field [0001] The invention relates to the technical field of sensors, in particular to a method for realizing one-step nucleic acid detection by using a liquid crystal sensor. Background technique [0002] The main principle of the liquid crystal nucleic acid sensor is to use the interaction of liquid crystal molecules-surfactant-nucleic acid aptamer-nucleic acid to be tested to affect the arrangement of liquid crystal molecules, thereby affecting its optical image under a polarizing microscope. When the nucleic acid to be tested is detected, the nucleic acid to be tested and the nucleic acid aptamer are connected by hydrogen bond interaction, and the surfactant is free and drives the liquid crystal molecules to form a vertical arrangement; when the nucleic acid to be tested is not detected, the nucleic acid aptamer and The surfactants are connected by electrostatic interactions, and the liquid crystal molecules are arranged in a chaotic manner. [0003] The exist...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/23G02F1/13
CPCG01N21/23G02F1/1313
Inventor 瞿瑞祥李国强
Owner ZHEJIANG LAB