Unlock instant, AI-driven research and patent intelligence for your innovation.

Method for realizing one-step nucleic acid detection by using liquid crystal sensor

A sensor and nucleic acid sensor technology, applied in the field of sensors, can solve the problems of reducing the detection efficiency of liquid crystal nucleic acid sensors, changing the optical image of the sensor, affecting the stability, etc., achieving the effects of fast detection rate, strong universality, and good stability

Active Publication Date: 2022-03-25
ZHEJIANG LAB
View PDF10 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In this three-step method, the competition between electrostatic interaction and hydrogen bond interaction takes a long time. On the one hand, the detection efficiency of the liquid crystal nucleic acid sensor is reduced. On the other hand, the optical image of the sensor is changed due to liquid phase volatilization, which affects its stability. sex

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for realizing one-step nucleic acid detection by using liquid crystal sensor
  • Method for realizing one-step nucleic acid detection by using liquid crystal sensor
  • Method for realizing one-step nucleic acid detection by using liquid crystal sensor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Embodiment 1, utilize liquid crystal sensor to realize the one-step method detection of SARS virus oligonucleotide

[0054] In this embodiment, the microgrid film used for preparing the liquid crystal sensor is a microgrid nickel mesh, and the liquid crystal used is E7 liquid crystal. After the E7 liquid crystal is perfused, the sensor is left at 90°C for 20 minutes to ensure that the E7 liquid crystal completely enters the grid of the micro-grid nickel mesh. During the detection process, the surfactant used is cetyltrimethylammonium bromide (CTAB), and the nucleic acid aptamer is a complementary DNA single strand of SARS virus oligonucleotide, and its sequence is 5'-GCA TCA CCG GAT GAT-3', the nucleic acid to be tested is a SARS virus oligonucleotide, and its sequence is 5'-AUC AUC CGG UGA UGC-3'. Such as figure 1 As shown, if the existing "three-step method" is used for detection, the detection process is complicated, the detection takes a long time, and the optical...

Embodiment 2

[0058] Embodiment 2, using liquid crystal sensor to realize one-step detection of new coronavirus oligonucleotide

[0059] In this embodiment, the microgrid film used to prepare the liquid crystal sensor is a microgrid copper mesh, and the liquid crystal used is E7 liquid crystal. After perfusion of E7 liquid crystals, the sensor was left at 80°C for 30 minutes to ensure that the E7 liquid crystals completely entered the microgrid grid. During the detection process, the surfactant used is dodecyltrimethylammonium bromide (DTAB), and the nucleic acid aptamer is a complementary DNA single strand of the new coronavirus oligonucleotide, and its sequence is 5'-GCA TCT CCT GAT GAG-3', the nucleic acid to be tested is a new coronavirus oligonucleotide, and its sequence is 5'-CUC AUC AGG AGA UGC-3'. If the existing "three-step method" is used for detection, the detection process is complicated, the detection takes a long time, and the optical image is unstable. Therefore, this embod...

Embodiment 3

[0063] Embodiment 3, using a liquid crystal sensor to realize the one-step detection of the new coronavirus oligonucleotide

[0064] In this embodiment, the microgrid film used to prepare the liquid crystal sensor is a microgrid copper mesh, and the liquid crystal used is E7 liquid crystal. After perfusion of E7 liquid crystals, the sensor was left at 80°C for 30 minutes to ensure that the E7 liquid crystals completely entered the microgrid grid. During the detection process, the surfactant used is dodecyltrimethylammonium bromide (DTAB), and the nucleic acid aptamer is a complementary DNA single strand of the new coronavirus oligonucleotide, and its sequence is 5'-GCA TCT CCT GAT GAG-3', the nucleic acid to be tested is a new coronavirus oligonucleotide, and its sequence is 5'-CUC AUC AGG AGA UGC-3'. If the existing "three-step method" is used for detection, the detection process is complicated, the detection takes a long time, and the optical image is unstable. Therefore, ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for realizing one-step nucleic acid detection by using liquid crystal sensors. The method comprises the following steps: S1, preparing a plurality of liquid crystal nucleic acid sensors; s2, determining the specific volume fraction of the surfactant solution required by detection; s3, adding a to-be-detected nucleic acid solution into the nucleic acid aptamer solution, and adding the surfactant solution with the specific volume fraction obtained in the step S2 to obtain a to-be-detected solution; and S4, adding the liquid to be detected into the liquid crystal nucleic acid sensor, observing an optical image, and recording a detection result. The method can solve the problems of long detection time and unstable optical image in the current three-step method detection process, can realize instantaneous detection, and has the advantages of high detection rate, good stability, high sensitivity and strong universality.

Description

technical field [0001] The invention relates to the technical field of sensors, in particular to a method for realizing one-step nucleic acid detection by using a liquid crystal sensor. Background technique [0002] The main principle of the liquid crystal nucleic acid sensor is to use the interaction of liquid crystal molecules-surfactant-nucleic acid aptamer-test nucleic acid to affect the arrangement of liquid crystal molecules, and then affect its optical image under a polarizing microscope. When the nucleic acid to be tested is detected, the nucleic acid to be tested and the nucleic acid aptamer are connected by hydrogen bonds, and the surfactant is free and drives the liquid crystal molecules to arrange vertically; when the nucleic acid to be tested is not detected, the nucleic acid aptamer and the The surfactants are connected by electrostatic interaction, and the liquid crystal molecules are arranged in disorder. [0003] Existing liquid crystal nucleic acid sensors...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N21/23G02F1/13
CPCG01N21/23G02F1/1313
Inventor 瞿瑞祥李国强
Owner ZHEJIANG LAB