Lavender composition as well as preparation method and application thereof

A composition, lavender technology, applied in the directions of pharmaceutical formulations, cosmetic preparations, cosmetic preparations, etc., can solve problems such as no clear activity reports, achieve outstanding tyrosinase inhibitory activity, reduce costs, and improve tyrosine. Effect of Enzyme Inhibitory Activity

Pending Publication Date: 2022-04-05
INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But there is no clear report on the activity of its composition with other extracts

Method used

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  • Lavender composition as well as preparation method and application thereof
  • Lavender composition as well as preparation method and application thereof
  • Lavender composition as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] The preparation of embodiment 1 lavender composition

[0018] (1) extracting the Herba Aucifera powder in 60% ethanol solution by percolation to obtain an ethanol extract, recovering the ethanol solvent to obtain the first extract; (2) adding a small amount of water to the first extract, Form a suspension, and adjust the pH value of the suspension between 4.5 and 6.0; (3) add an appropriate amount of cellulase to the suspension, and react in a water bath at 45°C for 60 minutes; (4) transfer the reaction solution to a Soxhlet extractor, and add 95% Ethanol, leached for 90 minutes, filtered and separated after cooling, and the filtrate was concentrated to prepare the extract of elixir (sample A); (5) the extract of elixir and lavender essential oil were mixed at a ratio of 100:5 (hereinafter referred to as sample 1).

Embodiment 2

[0019] Example 2 Comparison of different preparation methods of the extract of Azalea radix

[0020] Put the fenugreek powder in a 60% ethanol solution and extract it by percolation to obtain an ethanol extract, recover the ethanol solvent, and obtain an alcohol extraction sample, which is mixed with lavender essential oil at a ratio of 100:5 (hereinafter referred to as sample 2)

[0021] Put the powder of Herbs officinalis in 60% ethanol solution and use the percolation method to extract the ethanol extract, recover the ethanol solvent to obtain the first extract, extract the first extract with ethyl acetate, combine the extracts, and recover acetic acid Ethyl ester sample, ethyl acetate sample mixed with lavender essential oil at a ratio of 100:5 (hereinafter referred to as sample 3)

[0022] Put the Herbs officinalis powder in 60% ethanol solution and use the percolation method to extract the ethanol extract, recycle the ethanol solvent to obtain the first extract, extract ...

Embodiment 3

[0029] Embodiment 3 Different preparation methods tyrosinase activity comparison

[0030] As a person skilled in the art, everyone knows that the parts extracted by different extraction methods contain different compounds and have different activities. On the premise that all the chemical components are not clear, the activities of the samples prepared in Example 1 and Example 2 were compared through the tyrosine activity.

[0031] Determination of tyrosinase (TYR) activity: Measure 3 mL of 50 mmol / L PBS (pH=6.8), add 2.9 mL of 1 mmol / L levodopa solution, place in a 32°C water bath for 20 min, add Mix 0.1 mL of TYR solution (0.01 g / L), and measure the absorbance curve of the mixture with time under constant temperature conditions. It is stipulated that the absorbance value of the reaction solution at a wavelength of 475 nm increases by 0.001 per minute as a TYR activity unit (U / min), and kojic acid is used as a positive control.

[0032] Calculate the tyrosinase inhibition r...

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Abstract

The invention provides a lavender composition, a preparation method and application thereof, and belongs to the technical field of plant extracts, the lavender composition comprises the following components: 0.59 to 3.60 mg / g of apigenin, 2.08 to 4.22 mg / g of baicalein-7-methyl ether, 28.57 to 114.28 [mu] g / g of quercetin, 0.75 to 3.13 mg / g of kaempferol, 32.38 to 114.28 [mu] g / g of luteolin, 4.02 to 6.35 [mu] g / g of chrysoeriol, and 47.6 mg / g of lavender essential oil. The lavender composition shows prominent tyrosinase inhibition activity when the concentration is 0.2 mg / ml, the tyrosinase inhibition activity is far higher than 1 + 1 activity of the elsholtzia extract and the lavender essential oil, and the whitening effect can be achieved by inhibiting the tyrosinase activity.

Description

technical field [0001] The invention belongs to the technical field of plant extracts, and in particular relates to a whitening plant composition and its preparation method and application. Background technique [0002] With the improvement of people's living standards and aesthetic standards, smooth and fair skin is more and more respected by women, and the proportion of whitening products in the daily chemical product market is also increasing. Due to the safe and healthy performance of natural functional substances, daily chemical products containing natural plant whitening functional ingredients have gradually become the consumer objects that people respect and the product orientation of the industry. The commonly used commercialized natural whitening extract is glabra root extract , peony root extract and tea extract etc. [0003] Tyrosinase inhibitors from natural sources are attracting more and more attention due to their safety, effectiveness, and ability to effecti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/9789A61K8/92A61K8/63A61K8/49A61Q19/02
Inventor 韦敏汤毅汤承翰
Owner INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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