Cultivation method and application of mushroom penicilliosis strong-resistance strain

A cultivation method and green mildew technology are applied in the field of cultivating strong green mildew resistant strains of Lentinus edodes, and can solve problems such as lack of disease-resistant varieties and the like

Pending Publication Date: 2022-04-22
HUAZHONG AGRI UNIV +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] At present, the most effective way to prevent and control edible fungus green mold is to select disease-resistant varieties and control environmental sanitation. At present, in ad

Method used

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  • Cultivation method and application of mushroom penicilliosis strong-resistance strain
  • Cultivation method and application of mushroom penicilliosis strong-resistance strain
  • Cultivation method and application of mushroom penicilliosis strong-resistance strain

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Experimental program
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Effect test

Embodiment 1

[0057] Embodiment 1 Trichoderma dark viridans fermented liquid induces the induction effect of cultivating shiitake mushroom strain S1

[0058] (1) Preparation of deactivated Trichoderma dark viridans fermentation broth: use a 250mL Erlenmeyer flask to prepare potato dextrose liquid (PDA) medium, the bottle contains 200mL of medium, and sterilize under high pressure steam at 115°C for 30min. Punch a hole at the edge of the activated Trichoderma dark green colony to take about 8mm (1mL blue pipette tip, the same below) bacterial block, put it into the liquid medium, inoculate 3 bacterial blocks in each bottle, place Shake the flask in a constant temperature shaking incubator at 28°C in the dark for 24 hours before taking it out. Use a wire filter to separate the Trichoderma dark green mycelium ball and the culture solution, and use tweezers to take out the solid medium particles in the mycelium ball. Afterwards, filter the culture solution again with a funnel and double-circle...

Embodiment 2

[0065] Embodiment 2 Trichoderma dark viridans fermented liquid induces the inducing effect of cultivating shiitake mushroom strain S2

[0066] Inoculate the shiitake mushroom strain S2 after the activation culture on the center of the induction medium IMYG plate, and after three induction cultures, take the edge hyphae of the colony after the third induction culture (3C), and use the colony infection method to measure the mycelia of the strain S2 The change AAC of the antagonistic ability to the hyphae of Trichoderma dark green, the result shows that the AAC of strain S2 after induction 3 times is 42.13%, and its induction effect is shown in figure 2 .

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Abstract

The method comprises the following steps: obtaining a mushroom strain to be selected, carrying out induction culture for at least one time, carrying out resistance evaluation after each induction culture is finished, screening strains of which the antagonism ability change is greater than or equal to a preset threshold value, and determining that the strain has the strong resistance to the mushroom green mold according to the fact that the antagonism ability change is greater than or equal to the preset threshold value. The strain resistance is stronger; and screening suitable strains with strong resistance. The induction culture comprises the step of inoculating a single colony of the last-generation shiitake mushroom strain into an induction culture medium, and culturing until hyphae grow all over the culture medium; the induction culture medium contains deactivated trichoderma atroviride fermentation liquor for inducing a mushroom strain to improve the disease resistance of green mold; an induction culture medium containing 0.75-1.5% of inactivated trichoderma atroviride fermentation liquor is used for culturing for 1-5 times, so that the resistance of the strain can be effectively improved; the cultivation method of the mushroom green mold strong-resistance strain can be applied to prevention and treatment of bag-cultivated or bottle-cultivated edible mushroom diseases caused by trichoderma.

Description

technical field [0001] The invention belongs to the field of methods for cultivating strong resistant strains of edible fungi, and more specifically, relates to a method for cultivating strong resistant strains of mushroom green mold and its application. Background technique [0002] Trichoderma spp. is the main source of infection of edible fungus green mold disease, the common ones are T.cf.virensv VonArx, T.longibrachiatum Rifai, Trichoderma harzianum (T.harzianum Rifai), T.atrovide Karsten, T.asperellum Samuels, Lieckf&Nirenberg, etc. Trichoderma can directly pollute the culture material, or infect the fungus covered with mushroom mycelium The green mold caused by it is the most serious disease in the cultivation and production of shiitake mushrooms, which often brings serious losses in the early stage of shiitake mushroom cultivation and production. [0003] Chemical fungicides have a bactericidal effect on fungi, but both edible fungi and Trichoderma are fungi, and Tr...

Claims

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Application Information

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IPC IPC(8): A01G18/00A01G18/10
CPCA01G18/00A01G18/10Y02W30/40
Inventor 边银丙郭孟配杜捷肖扬马晓龙
Owner HUAZHONG AGRI UNIV
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