Cultivation method and application of mushroom penicilliosis strong-resistance strain

A cultivation method and green mildew technology are applied in the field of cultivating strong green mildew resistant strains of Lentinus edodes, and can solve problems such as lack of disease-resistant varieties and the like
CN114375763APending Publication Date: 2022-04-22HUAZHONG AGRI UNIV +1
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
HUAZHONG AGRI UNIV
Publication Date
2022-04-22

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Abstract

The method comprises the following steps: obtaining a mushroom strain to be selected, carrying out induction culture for at least one time, carrying out resistance evaluation after each induction culture is finished, screening strains of which the antagonism ability change is greater than or equal to a preset threshold value, and determining that the strain has the strong resistance to the mushroom green mold according to the fact that the antagonism ability change is greater than or equal to the preset threshold value. The strain resistance is stronger; and screening suitable strains with strong resistance. The induction culture comprises the step of inoculating a single colony of the last-generation shiitake mushroom strain into an induction culture medium, and culturing until hyphae grow all over the culture medium; the induction culture medium contains deactivated trichoderma atroviride fermentation liquor for inducing a mushroom strain to improve the disease resistance of green mold; an induction culture medium containing 0.75-1.5% of inactivated trichoderma atroviride fermentation liquor is used for culturing for 1-5 times, so that the resistance of the strain can be effectively improved; the cultivation method of the mushroom green mold strong-resistance strain can be applied to prevention and treatment of bag-cultivated or bottle-cultivated edible mushroom diseases caused by trichoderma.
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Description

technical field

[0001] The invention belongs to the field of methods for cultivating strong resistant strains of edible fungi, and more specifically, relates to a method for cultivating strong resistant strains of mushroom green mold and its application. Background technique

[0002] Trichoderma spp. is the main source of infection of edible fungus green mold disease, the common ones are T.cf.virensv VonArx, T.longibrachiatum Rifai, Trichoderma harzianum (T.harzianum Rifai), T.atrovide Karsten, T.asperellum Samuels, Lieckf&Nirenberg, etc. Trichoderma can directly pollute the culture material, or infect the fungus covered with mushroom mycelium The green mold caused by it is the most serious disease in the cultivation and production of shiitake mushrooms, which often brings serious losses in the early stage of shiitake mushroom cultivation and production.

[0003] Chemical fungicides have a bactericidal effect on fungi, but both edible fungi and Trichoderma are fungi, and Tr...

Claims

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