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Method for constructing liver injury animal model after limb ischemia reperfusion

An ischemia-reperfusion and animal model technology, applied in the field of construction of animal models of liver injury after limb ischemia-reperfusion, can solve the problems of high reproducibility, high success rate, model stability, etc., and achieve easy operation and promotion, modeling The method is simple and the effect of balancing oxygen free radicals

Pending Publication Date: 2022-04-22
李岩
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Problems solved by technology

So far, there is no study on the evaluation criteria of the rat model of liver injury after limb ischemia-reperfusion, and there is no method with good reproducibility, high success rate, and good model stability

Method used

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Embodiment

[0021] The present invention will be further described below in conjunction with specific examples.

[0022] A method for constructing an animal model of liver injury after limb ischemia-reperfusion of the present invention; comprising the following steps:

[0023] 1) Animal grouping and feeding: 10 SPF grade SD rats with a body weight of 150-200 g were selected, fed with a rat maintenance feed that met the national standard for one week, and water was deprived for 2 hours before modeling. Provided by the center (use license number: SYXK (Gan) 2015-0005, ethics review number: (Gan) 2018-102); animal breeding conditions: natural lighting according to circadian rhythm, rearing in an SPF-level experimental animal barrier system, laboratory temperature Keep at 20-25°C, relative humidity 50%-65%, keep the environment quiet and prevent noise; animals drink water and eat freely, and after one week of adaptive feeding, they are randomly divided into model group (10 rats) and normal gr...

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Abstract

The invention relates to a construction method of a liver injury animal model after limb ischemia reperfusion, which comprises the following steps: taking 10 SPF-grade SD (Sprague Dawley) rats with the weight of 150-200g, feeding the rats for one week, and forbidding water 2 hours before modeling. The method comprises the following steps: performing intraperitoneal anesthesia on a rat by using 1% pentobarbital sodium (25mg / kg), constraining four limbs and the head on a self-made rat plate, removing hair from a left rear leg, cerclaging a tourniquet at the root of a thigh, blocking for 2 hours, loosening the tourniquet, continuing for 6 hours, sampling 5mL of blood from the heart, centrifuging whole blood, sub-packaging serum, and storing at-20 DEG C. And detecting ALT, AST, SOD, MDA and GSH indexes of serum. Laparotomy is carried out to take the liver, the liver is put into frozen normal saline to be repeatedly rinsed, and finally the liver index is calculated. The method has the beneficial effects that the modeling time is short, the success rate is high, and the replicability is good; the method is applied to a series of disaster medical researches, has a wide application prospect, and can generate good social benefits, economic benefits and scientific research values.

Description

technical field [0001] The invention relates to the technical field of animal model construction, in particular to a method for constructing an animal model of liver injury after limb ischemia-reperfusion. Background technique [0002] With the rapid development of modern society, more and more means of transportation have realized high-speed and convenient transfer of people to engage in various social communication and economic activities. During this period, in addition to casualties caused by various traffic accidents or accidents, there were also many drivers and passengers whose limbs were trapped due to the change of vehicle shape. Long-term limb compression will inevitably lead to limb ischemia. When the ambulance personnel arrived, The moment the injured person is rescued from the deformed vehicle, the compression of the distal limbs is released, the blood flow is restored, and the anaerobic metabolites and a large number of inflammatory factors in the limbs caused ...

Claims

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Application Information

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IPC IPC(8): A01K67/02A61K49/00
CPCA01K67/02A61K49/0008A01K2207/30A01K2227/105A01K2267/03
Inventor 李岩刘娅楠康万荣张娜
Owner 李岩
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