Method for constructing liver injury animal model after limb ischemia reperfusion
An ischemia-reperfusion and animal model technology, applied in the field of construction of animal models of liver injury after limb ischemia-reperfusion, can solve the problems of high reproducibility, high success rate, model stability, etc., and achieve easy operation and promotion, modeling The method is simple and the effect of balancing oxygen free radicals
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[0021] The present invention will be further described below in conjunction with specific examples.
[0022] A method for constructing an animal model of liver injury after limb ischemia-reperfusion of the present invention; comprising the following steps:
[0023] 1) Animal grouping and feeding: 10 SPF grade SD rats with a body weight of 150-200 g were selected, fed with a rat maintenance feed that met the national standard for one week, and water was deprived for 2 hours before modeling. Provided by the center (use license number: SYXK (Gan) 2015-0005, ethics review number: (Gan) 2018-102); animal breeding conditions: natural lighting according to circadian rhythm, rearing in an SPF-level experimental animal barrier system, laboratory temperature Keep at 20-25°C, relative humidity 50%-65%, keep the environment quiet and prevent noise; animals drink water and eat freely, and after one week of adaptive feeding, they are randomly divided into model group (10 rats) and normal gr...
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