Artificial spawning induction and hatching method for spinibarbus hollandi

A technology of Hastelloy's square mouth and artificial induction of labor, which can be used in pharmaceutical formulations, climate change adaptation, medical preparations with inactive ingredients, etc.

Active Publication Date: 2022-04-29
西双版纳云博水产养殖开发有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the effect of the existing oxytocic agents takes a long time, and the effect usually takes more than ten hours, and the oxytocic agents made of these hormone analogs will be quickly eliminated by metabolism in the body, and the fish are directly injected with the oxytonic agents The time for the effect of the drug to appear has not yet come, and most of the oxytocic agents are metabolized and eliminated in the body, requiring repeated injections, which is very irritating to fish and wastes drugs

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1: Preparation of Cortex Moutan Extract.

[0028] Get 50kg peony root bark and pulverize it, and then pass through an 80-mesh sieve for subsequent use. Take the sieved peony root bark fine powder, add water, and extract under reflux at a temperature of 90-100° C. for 24 hours. After the extraction is completed, the extract is concentrated under reduced pressure with a rotary evaporator to obtain the Cortex Moutan extract, which is stored away from light.

Embodiment 2

[0029] Embodiment 2: A method for artificially inducing spawning and hatching of squaremouth bass.

[0030] Step S1. Parental selection: select mature broodstock for breeding, and separate the female square-mouthed harveyer and the male harveyer's for a while in a pool, the temperature in the pool is 24-28°C, and the dissolved oxygen is kept at 8 mg / Above L, the water velocity is 0.2-0.6m / s;

[0031] Step S2. Artificial oxytocin: prepare a slow-release catalyst, prepare PG, HCG, the Moutan Cortex extract obtained in Example 1, and polyglycolide with a number average molecular weight of 80,000 g / mol with 0.5% saline by mass fraction PG, HCG, the Cortex Moutan extract prepared in Example 1, and the polyglycolide with a number average molecular weight of 80000g / mol are respectively 2mg / g, 100IU / g, 4μg / g, 4mg in physiological saline / g, respectively inject slow-release oxytocic agent to female and male square-mouthed catfish, inject 0.5-1g slow-release oxytocic agent per kilogra...

Embodiment 3

[0034] Embodiment 3: A method for artificially inducing spawning and hatching of squaremouth bass.

[0035] Step S1. Parental selection: select mature broodstock for breeding, and separate the female square-mouthed harveyer and the male harveyer's for a while in a pool, the temperature in the pool is 24-28°C, and the dissolved oxygen is kept at 8 mg / Above L, the water velocity is 0.2-0.6m / s;

[0036] Step S2. Artificial oxytocin: Prepare a slow-release catalyst, prepare PG, HCG, the Moutan cortex extract obtained in Example 1, and polyglycolide with a number average molecular weight of 90,000 g / mol with 0.5% saline by mass fraction PG, HCG, the Cortex Moutan extract prepared in Example 1, and the polyglycolide with a number average molecular weight of 90000g / mol are respectively 2mg / g, 100IU / g, 4μg / g, 4mg in physiological saline / g, respectively inject slow-release oxytocic agent to female and male square-mouthed catfish, inject 0.5-1g slow-release oxytocic agent per kilogra...

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Abstract

The invention discloses an artificial spawning induction and hatching method for barbus hollandi, which comprises the following steps: S1, parent selection: selecting mature breeding parent fish, and separately and temporarily culturing female barbus hollandi and male barbus hollandi in a water pool; s2, artificial spawning induction, wherein slow-release oxytocic is injected to the female spinibarbus hollandi and the male spinibarbus hollandi respectively, and the slow-release oxytocic comprises PG, HCG, a moutan bark extract and poly (lactic-co-glycolic acid); s3, artificial insemination: after artificial spawning induction is completed, performing flowing water stimulation on the female barbus hollandi and the male barbus hollandi, observing the activity conditions of the female barbus hollandi and the male barbus hollandi, and starting artificial insemination when the male barbus hollandi collides with the female barbus hollandi and eggs appear in water to obtain fertilized eggs; s4, artificial incubation: adjusting water temperature, pH, dissolved oxygen and water flow velocity, and incubating fertilized eggs. The method can effectively shorten the effective time of the oxytocic, reduce the injection frequency, relieve the stimulation to the fish and improve the fertilization rate and the hatching rate.

Description

technical field [0001] The invention belongs to the field of aquaculture, and in particular relates to a method for artificially inducing spawning and hatching of squid harveyer. Background technique [0002] Hashi's squaremouth belongs to the Cyprinidae family and is a rare and endangered indigenous fish in the Lancang River. Barb has tender meat, delicious taste, high nutritional value, and high economic value. At present, barb has only a small amount of artificial breeding in Yunnan Province, and the artificial reproduction technology is difficult. The main reason is that the female ha’s The amount of ovulation in the barb was low, and the male barb had a small amount of semen and the duration of ejaculation was short, which affected the quality of fertilization. [0003] At present, in order to solve the problem of less ovulation of fish females or less sperm production of males, oxytocic injections are usually used to induce labor. Commonly used oxytocic agents includ...

Claims

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Application Information

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IPC IPC(8): A01K61/10A01K61/17A61K38/24A61K47/59A61K47/51A61P15/08A61K38/22
CPCA01K61/10A01K61/17A61K38/24A61K38/22A61K47/593A61K47/51A61P15/08A61K2300/00Y02A40/81
Inventor 王敏奇王云清李燕李怡凌石凤德黄绍刚普忠南
Owner 西双版纳云博水产养殖开发有限公司
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