Aluminum nanocrystal composite immune drug as well as preparation method and application thereof

An immune drug, aluminum nanotechnology, applied in the field of biomedicine, can solve the problems of lack of targeting, adverse effects of embryonic tissue, etc., and achieve the effect of improving immune response, inhibiting growth, and enhancing stability

Pending Publication Date: 2022-05-24
THE GBA NAT INST FOR NANOTECHNOLOGY INNOVATION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, systemic administration of cytokines has certain systemic toxicity due to lack of targeting, and has potential adverse effects on embryonic tissues. Therefore, local administration of cytokine drugs is a relatively effective and safe way

Method used

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  • Aluminum nanocrystal composite immune drug as well as preparation method and application thereof
  • Aluminum nanocrystal composite immune drug as well as preparation method and application thereof
  • Aluminum nanocrystal composite immune drug as well as preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0034] AlCl 3 ·6H 2 O was dissolved in 0.01 mol / L NaAc, and prepared into an aluminum salt solution with an aluminum ion content of 0.05 mol / L; PS-PEG and M-PEG-NH with a total molecular concentration of 0.05 mol / L were added to the obtained aluminum salt solution. 2 A mixed solution with a molar ratio of 1:1 in which Al 3+ The molar ratio between phosphoserine and phosphoserine is 1:2; after the reaction is completed, stand for 7 hours, wash after centrifugation, and finally sterilize by high pressure steam or sterilize through a filter membrane to obtain aluminum nanocrystals, which are named Al-PEG.

Embodiment 2

[0035] Example 2 Preparation of aluminum nanocrystal composite immune drug Al-RGD-IL12:

[0036] (1) Add aluminum nanocrystals and the polypeptide sequence c (RGDfC) that can specifically bind to the highly expressed functional protein integrin on the surface of tumor cells into 0.1M phosphate buffer or 0.1M ammonium acetate buffer, wherein the polypeptide sequence and aluminum The ratio of maleimide contained in PEG molecules on the surface of nanocrystals was 1.2:1, and the reaction was stirred for 24 hours, and then centrifuged, washed and purified to obtain aluminum nanocrystals modified with RGD peptide sequence;

[0037](2) The cytokine IL-12 molecule was added to 50mM MES buffer, and EDC / NHS was added to activate the carboxyl group on the molecule, and then the solution of nanocrystals obtained in step (1) was added, wherein the surface of the aluminum nanocrystals was M-PEG- NH 2 The ratio of 1.5:1 to cytokine molecules was obtained, and the aluminum nanocrystal compo...

Embodiment 3

[0038] Example 3 Aluminum nanocrystal composite immune drug Al-TAT-TNFα:

[0039] (1) Add the aluminum nanocrystals and the transmembrane peptide sequence TAT, which can effectively carry the nanoparticles into the cell, into 0.1M phosphate buffer or 0.1M ammonium acetate buffer, wherein the polypeptide sequence and the PEG molecule on the surface of the aluminum nanocrystal are added. The ratio of maleimide contained is 1.5:1, the reaction is stirred for 24 hours, and centrifuged, washed and purified to obtain aluminum nanocrystals modified with TAT peptide sequence;

[0040] (2) Add tumor necrosis factor TNFα molecule into 50mM MES buffer, and add EDC / NHS to activate the carboxyl group on the molecule, and then add the solution of nanocrystals obtained in step (1), wherein the surface of aluminum nanocrystals is M-PEG-NH 2 The ratio of cytokine molecules to cytokine molecules is 1.3:1, and an aluminum nanocrystal composite immune drug further modified by cytokine molecules i...

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Abstract

The invention relates to the technical field of biological medicines and vaccines, in particular to an aluminum nanocrystal composite immune drug as well as a preparation method and application thereof. Wherein the aluminum nanocrystal is used as a carrier, the surface of the aluminum nanocrystal is covered with polyethylene glycol, and a functional polypeptide sequence and cytokine molecules are linked through a polyethylene glycol terminal group, so that a virus-like particle immune drug is formed, the stability of cytokines is improved, the retention time of the cytokines in tumor tissues is prolonged, and the auxiliary antitumor effect of the cytokines is effectively improved.

Description

technical field [0001] The invention relates to the field of biomedical technology and vaccine technology, in particular to an aluminum nanocrystal composite immune medicine and a preparation method and application thereof. Background technique [0002] Up to now, although the theoretical research and related technologies in clinical medicine have made great progress, tumor (cancer) is still a disease that is difficult to cure and has a high fatality rate. Clinically, there are three traditional treatment options for tumors: surgery, chemotherapy and radiation. Surgical therapy, which directly removes the diseased site through surgery, is effective for early-stage tumors or benign tumors with low spreading ability, but for cancer patients that have metastasized, its efficacy and prognosis are poor. Due to the lack of specificity of chemotherapy, while treating tumors, it also does great harm to the patient's own immune system and stem cells. In recent years, with the conti...

Claims

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Application Information

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IPC IPC(8): A61K47/52A61K47/60A61K47/69A61K38/12A61K38/20A61K38/06A61K38/19A61P35/00
CPCA61K47/52A61K47/60A61K47/6935A61K38/12A61K38/208A61K38/06A61K38/191A61K9/0019A61P35/00A61K2300/00
Inventor 王亚玲陈春英赵宇亮
Owner THE GBA NAT INST FOR NANOTECHNOLOGY INNOVATION
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