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Preparation method and application of fluorogold nano-cluster coupled Napsin A compound

A technology of fluorescent gold nanometers and complexes, which is applied in the field of preparation of fluorescent gold nanoclusters coupled with NapsinA complexes, can solve problems such as failure to identify target lesions, expand the scope of surgical resection, etc., and achieve the effect of uniform size

Pending Publication Date: 2022-05-27
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the characteristics of pulmonary ground-glass nodules, target identification often fails during thoracoscopic surgery. Traditional intraoperative anatomical marker positioning and CT-guided percutaneous auxiliary positioning have their limitations, resulting in conversion to thoracotomy. Or extended surgical resection often occurs

Method used

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  • Preparation method and application of fluorogold nano-cluster coupled Napsin A compound
  • Preparation method and application of fluorogold nano-cluster coupled Napsin A compound
  • Preparation method and application of fluorogold nano-cluster coupled Napsin A compound

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Add 16 mg of 1-ethyl-(3-dimethylaminopropyl)carbodiimide and 10 mg of N-hydroxysuccinimide to a flask containing 10 mL of N,N-dimethylformamide and stir for a few minutes Then add 15mg 3-mercaptopropionic acid, continue stirring for 30min, add 1mL 100mg / mL polyethyleneimine solution into the flask dropwise with vigorous stirring, and stir for 12h;

[0027] The mixed solution was rotary-evaporated, reverse-precipitated, and centrifuged, and the collected precipitate was dispersed into 1 mL of water, 0.1 mL of which was added to 5 mL of water, and then 0.1 mL of chloroauric acid solution and 0.3 mL of hydrazine hydrate were added. , heated for 4h, the reaction solution changed from light yellow to brown;

[0028] The synthesized gold nanoclusters were lyophilized by dialysis for 24 h, 10 μL of antibody was added to 1 mL of TPBS buffer solution, 14.4 mg of 1-ethyl-(3-dimethylaminopropyl) carbodiimide, 4.8 mg of N- Hydroxysuccinimide was activated by stirring at room tempe...

Embodiment 2

[0032] 32 mg of 1-ethyl-(3-dimethylaminopropyl)carbodiimide and 20 mg of N-hydroxysuccinimide were added to a flask containing 20 mL of N,N-dimethylformamide and stirred for a few minutes Then add 30mg 3-mercaptopropionic acid, continue stirring for 30min, add 2mL 100mg / mL polyethyleneimine solution into the flask dropwise with vigorous stirring, and stir for 24h;

[0033] The mixed solution was rotary evaporated, reverse-precipitated and centrifuged, the collected precipitate was dispersed in 2 mL of water, 0.3 mL of it was added to 5 mL of water, 0.3 mL of chloroauric acid solution and 0.6 mL of hydrazine hydrate were added, and the mixture was stirred rapidly at 90 °C. , heated for 8h, the reaction solution changed from light yellow to brown;

[0034] The synthesized gold nanoclusters were lyophilized by dialysis for 24 h, 30 μL of antibody was added to 3 mL of TPBS buffer solution, 32 mg of 1-ethyl-(3-dimethylaminopropyl) carbodiimide, 10 mg of N-hydroxysuccinic acid were ...

Embodiment 3

[0038] 64 mg of 1-ethyl-(3-dimethylaminopropyl)carbodiimide and 40 mg of N-hydroxysuccinimide were added to a flask containing 40 mL of N,N-dimethylformamide and stirred for a few minutes Then add 60mg 3-mercaptopropionic acid, continue stirring for 30min, add 5mL 100mg / mL polyethyleneimine solution into the flask dropwise with vigorous stirring, and stir for 48h;

[0039] The mixed solution was rotary evaporated, reverse-precipitated and centrifuged, the collected precipitate was dispersed in 5 mL of water, 0.5 mL of it was added to 5 mL of water, 0.5 mL of chloroauric acid solution and 1.2 mL of hydrazine hydrate were added, and the mixture was stirred rapidly at 90 °C. , heated for 12h, the reaction solution changed from light yellow to brown;

[0040] The synthesized gold nanoclusters were lyophilized by dialysis for 48 h, 50 μL of antibody was added to 5 mL of TPBS buffer solution, 64 mg of 1-ethyl-(3-dimethylaminopropyl) carbodiimide, 20 mg of N-hydroxysuccinic acid were...

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Abstract

The invention relates to the technical field of high polymer materials, in particular to a preparation method and application of a fluorogold nanocluster coupled Napsin A compound. Comprising the following steps: adding 1-ethyl-(3-dimethylaminopropyl) carbodiimide and N-hydroxysuccinimide into a flask of N, N-dimethylformamide, stirring for several minutes, adding 3-mercaptopropionic acid, continuously stirring, and dropwise adding an amino-containing high-molecular long-chain polymer solution under the condition of violent stirring; according to the preparation method and the application of the fluorescent gold nano-cluster coupled Napsin A compound, bright red fluorescent gold nano-clusters are prepared, then 1-ethyl-(3-dimethylaminopropyl) carbodiimide / N-hydroxysuccinimide is utilized for reaction to be coupled with a Napsin A antibody, the compound with a targeting function is obtained, and early diagnosis and intraoperative positioning of lung cancer are carried out.

Description

technical field [0001] The invention relates to the technical field of polymer materials, in particular to a preparation method and application of a fluorescent gold nanocluster coupled Napsin A complex. Background technique [0002] With the wide application of high-resolution CT in lung cancer screening, the detection rate of ground-glass nodules in early-stage lung cancer is increasing. For small pulmonary nodules, traditional inspection methods such as needle biopsy, bronchoscopy, and positron emission tomography -X-ray computed tomography (PET-CT) is difficult to diagnose. The proportion of pulmonary nodules in the spectrum of thoracic surgery diseases is increasing year by year. Thoracoscopic surgery has the advantages of minimally invasive and rapid recovery, and is the first choice for surgery for patients with pulmonary ground glass nodules. However, due to the characteristics of pulmonary ground glass nodules, target identification during thoracoscopic surgery of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/00
CPCA61K49/0019A61K49/0056
Inventor 汤璐林权王跃
Owner JILIN UNIV
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