Gossypium hirsutum GhEXO2 and application thereof in plant type regulation
A technology of upland cotton, plant type, applied in the fields of application, climate change adaptation, chemical instruments and methods, etc.
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Embodiment 1
[0022] Example 1, as image 3 As shown, GhEXO2 transgenic Arabidopsis leaf phenotype identification analysis and possible regulatory network analysis:
[0023] In order to understand the biological function of GhEXO2, GhEXO2 was ectopically overexpressed in Arabidopsis by constructing an overexpression vector.
[0024] The primer list is shown in Table 1 below:
[0025] Primer List 1
[0026]
[0027] Observing the phenotype at the seedling stage, it was found that the GhEXO2 transgenic lines had enhanced vegetative growth compared with the WT plants, just like AtEXO. The leaf area was measured, and it was found that the leaf area of the GhEXO2 transgenic plants was significantly larger than that of the WT plants, which was two of the WT plants. double ( image 3 shown in A and B).
[0028] In addition, through qRT-PCR and semi-quantitative RT-PCR analysis, it was confirmed again that GhEXO2 was relatively expressed in GhEXO2 transgenic plants ( image 3 C and D), in...
Embodiment 2
[0032] Example 2, as Figure 4 As shown, the use of VIGS technology to silence and verify that cotton GhEXO2 gene regulates cotton leaf size and plant height:
[0033] In order to further study the role of GhEXO2 in the regulation of cotton leaf shape, the pTRV system was used to knock down its expression by virus-induced gene silencing (VIGS) to verify the function of GhEXO2 in cotton.
[0034] As predicted, GhEXO2 expression was significantly suppressed and vegetative growth was reduced in silenced plants compared to controls ( Figure 4 A), leaf size changes ( Figure 4 B).
[0035] Statistical analysis found that the leaf area index (LAI) of the three independent lines of GhEXO2 silenced plants was significantly lower than that of the control ( Figure 4 E). qRT-PCR analysis showed that the observed expression of GhEXO2 was significantly reduced in the silenced lines compared to the control, indicating that GhEXO2 was efficiently silenced in the VIGS lines ( Figure 4...
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