Gossypium hirsutum GhEXO2 and application thereof in plant type regulation

A technology of upland cotton, plant type, applied in the fields of application, climate change adaptation, chemical instruments and methods, etc.

Active Publication Date: 2022-05-31
INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] There is no report in the prior art about the function and application of the upland cotton homologous gene GhEXO2 in regulating the leaf development of upland cotton. There is no report about the function of this gene in cotton. Therefore, studying the function of EXO in the development of cotton leaves is very important for exploring cotton The mechanism of leaf development and the development of ideal plant type are of great significance

Method used

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  • Gossypium hirsutum GhEXO2 and application thereof in plant type regulation
  • Gossypium hirsutum GhEXO2 and application thereof in plant type regulation
  • Gossypium hirsutum GhEXO2 and application thereof in plant type regulation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1, as image 3 As shown, GhEXO2 transgenic Arabidopsis leaf phenotype identification analysis and possible regulatory network analysis:

[0023] In order to understand the biological function of GhEXO2, GhEXO2 was ectopically overexpressed in Arabidopsis by constructing an overexpression vector.

[0024] The primer list is shown in Table 1 below:

[0025] Primer List 1

[0026]

[0027] Observing the phenotype at the seedling stage, it was found that the GhEXO2 transgenic lines had enhanced vegetative growth compared with the WT plants, just like AtEXO. The leaf area was measured, and it was found that the leaf area of ​​the GhEXO2 transgenic plants was significantly larger than that of the WT plants, which was two of the WT plants. double ( image 3 shown in A and B).

[0028] In addition, through qRT-PCR and semi-quantitative RT-PCR analysis, it was confirmed again that GhEXO2 was relatively expressed in GhEXO2 transgenic plants ( image 3 C and D), in...

Embodiment 2

[0032] Example 2, as Figure 4 As shown, the use of VIGS technology to silence and verify that cotton GhEXO2 gene regulates cotton leaf size and plant height:

[0033] In order to further study the role of GhEXO2 in the regulation of cotton leaf shape, the pTRV system was used to knock down its expression by virus-induced gene silencing (VIGS) to verify the function of GhEXO2 in cotton.

[0034] As predicted, GhEXO2 expression was significantly suppressed and vegetative growth was reduced in silenced plants compared to controls ( Figure 4 A), leaf size changes ( Figure 4 B).

[0035] Statistical analysis found that the leaf area index (LAI) of the three independent lines of GhEXO2 silenced plants was significantly lower than that of the control ( Figure 4 E). qRT-PCR analysis showed that the observed expression of GhEXO2 was significantly reduced in the silenced lines compared to the control, indicating that GhEXO2 was efficiently silenced in the VIGS lines ( Figure 4...

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Abstract

The invention discloses a cotton GhEXO2 gene, the nucleotide sequence of an encoding protein of the cotton GhEXO2 gene is shown as SEQ ID NO: 2, and the amino acid sequence of the encoding protein is shown as SEQ ID NO: 1. The invention belongs to the technical field of plant gene functions, and particularly provides a cotton GhEXO2 gene obtained by cloning for the first time, expression characteristic analysis is carried out, the function of the gene is verified in plants (arabidopsis thaliana and cotton) through a transgenic technology, and the cotton GhEXO2 gene has important practical significance in further understanding of the function of EXO and cultivation of ideal plant types of cotton.

Description

technical field [0001] The invention belongs to the technical field of cotton germplasm resource cultivation and molecular biology, in particular to cotton GhEXO2 (Gh_A04G0976) and its application in regulating leaf development and then affecting plant type. Background technique [0002] The ideal plant type is the morphological characteristic of high yield quality. The ideal plant type required by different crops is not exactly the same, but it mostly involves plant height, leaf shape, leaf posture, leaf color, leaf distribution, and the relationship between tiller and main stem. Leaves are the main photosynthetic organs of plants, have a significant impact on growth and development, and have a surprisingly diverse shape due to differences in cell expansion rates. Flexibility in leaf shape depends primarily on the size and arrangement of leaf margin growths. [0003] However, the physiological functions arising from different leaf shapes in plants are rarely reported. In...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C07K14/415C12N15/83A01H5/12A01H6/20A01H6/60
CPCC07K14/415C12N15/8218C12N15/8261Y02A40/146
Inventor 杨作仁李付广李胜刘钊王刚鲁丽丽章莲
Owner INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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