Method for synchronously and quantitatively measuring concentrations of various aromatic compounds and metabolites in urine
A technology for aromatic compounds and metabolites, applied in measuring devices, general water supply conservation, instruments, etc. It can shorten the required time, reduce matrix interference, and simplify the sample pretreatment steps.
Pending Publication Date: 2022-05-31
GUANGDONG UNIV OF TECH
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Problems solved by technology
Summarizing the prior art finds the following deficiencies: 1) the properties of monocyclic and polycyclic aromatic compounds are quite different, and there is a lack of an analytical method for the comprehensive simultaneous analysis and detection of these two types of aromatic compounds; 2) relevant polycyclic aromatic hydrocarbons derivatives The analysis method of pollutants stays in the analysis of pollutant prototypes in media such as blood
[0004] For the simultaneous quantitative method of multiple types of aromatic compounds and metabolites in urine, the difficulties lie in: 1) There are a wide variety of aromatic compounds, ranging from monocyclic to polycyclic, and the different types and quantities of substituents on the benzene ring lead to physical and chemical differences between substances. Due to the large difference in properties, it is one of the key technical issues to select a suitable extraction solvent to simultaneously extract multiple types of target substances; 2) There are many types of substances in liquid chromatography, it is difficult to completely separate each compound, and a single compound is affected by the total amount of substances , the sensitivity of mass spectrometry will decrease
Method used
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Embodiment 1
Embodiment 2
[0046] The present embodiment adopts the matrix urine sample to carry out the recovery experiment. That is, 30 urine samples were randomly collected, mixed and
[0051] REC=C
[0053] REC refers to the recovery rate (%) of the matrix spiked sample;
[0054] C
[0055] C
[0056] C
[0057] C
[0058] V
[0059] V
Embodiment 3
[0064] The present embodiment adopts the matrix urine sample (preparation method is the same as Example 2) to carry out a recovery rate experiment. This example adopts
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Abstract
The invention belongs to the technical field of analysis of toxic organic pollutants, and discloses a method for synchronously and quantitatively determining the concentration of various aromatic compounds and metabolites in urine. The method comprises the following steps: sequentially adding an isotope internal standard substance, a buffer solution and beta-glucuronidase-aryl sulfatase into the urine, carrying out constant-temperature enzymolysis, adding an organic solvent after enzymolysis, extracting, centrifuging, transferring supernatant, concentrating to a constant volume, adding into a polar solution, and carrying out quantification and data processing to obtain the concentration level of various target substances in the urine. By optimizing the scheme, synchronous detection and analysis of various aromatic compounds and metabolites with large property differences are realized, the experimental steps are simplified, the cost is reduced, the sensitivity is improved, and the method can be effectively applied to detection and analysis of large-batch urine samples.
Description
A Simultaneous Quantitative Determination of Multiple Aromatic Compounds and Metabolites in Urine Methods technical field The invention belongs to the technical field of toxic organic pollutant analysis, particularly a kind of simultaneous quantitative determination of polyoxymethylene in urine. Methods for Aromatic-Like Compounds and Metabolite Concentrations. Background technique Aromatic compounds, such as monocyclic compounds such as nitrophenol, chlorophenol, polycyclic compounds such as polycyclic aromatic hydrocarbons and derivatives thereof etc., are ubiquitous pollutants in the environment, mainly from incomplete combustion of fossil fuels, pesticide residues and industrial waste gas. in water waste. Long-term exposure to aromatic compounds may increase the risk of disease, such as exposure to nitrophenols Damage to the nervous system and organs such as liver and kidneys; high exposure to PCP may increase the risk of spontaneous abortion; long-term exposu...
Claims
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IPC IPC(8): G01N30/02G01N30/72G01N30/06
CPCG01N30/02G01N30/72G01N30/06G01N2030/062Y02A20/20
Inventor安太成李海玲姚春阳张书李桂英
OwnerGUANGDONG UNIV OF TECH