Nanobiomaterial, its preparation method and nucleic acid compound

A technology of biological nanomaterials and complexes, applied in the field of nucleic acid complexes, can solve the problems of low transfection efficiency and poor safety, and achieve the effects of improving transfection efficiency, reducing the proportion of degraded and good uniformity

Active Publication Date: 2013-01-23
NANKAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0010] However, they still have the problems of low transfection efficiency and poor safety

Method used

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  • Nanobiomaterial, its preparation method and nucleic acid compound
  • Nanobiomaterial, its preparation method and nucleic acid compound
  • Nanobiomaterial, its preparation method and nucleic acid compound

Examples

Experimental program
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preparation example Construction

[0024] The invention provides a method for preparing a bionano material, which is characterized in that the method comprises: selecting a nucleic acid of a required length and assembling and incubating the nucleic acid with a protein to obtain the bionano material, the nucleic acid can be initially combined with the The assembly between the proteins, the condition of the assembly incubation makes the nucleic acid wrapped by the protein, so that the nuclease cannot contact the nucleic acid through the protein that wraps the nucleic acid; wherein, the length of the nucleic acid is the same as that of the biological nanomaterial There is a linear relationship between the particle sizes, and the method for selecting the nucleic acid of the required length includes determining the required length of the nucleic acid according to the particle size of the desired bionano material.

[0025] The core of the present invention is to select a suitable system. In this system, nucleic acid a...

preparation example 1

[0067] This preparation example is used for preparing tobacco mosaic virus capsid protein

[0068] TMV shell protein was prepared by Fraenkel-Conrat's glacial acetic acid method. Add 2 times the volume of glacial acetic acid to the TMV virus liquid stored at 4°C (virus content 1-5mg / mL), shake at 4°C for 1-2 hours, centrifuge at 10,000×g for 30min to remove the RNA precipitate, take the supernatant and add Volume distilled water, dialysis with double distilled water in 4°C refrigerator for 2-3 days, change the water 3-4 times a day, when TMV-CP is close to the isoelectric point (pH4 or so), it starts to coagulate, add a few drops of 3mol / L NaAcO buffer solution (pH4.7) to make the precipitation complete. Centrifuge at 12000rpm for 30min, dissolve the precipitate with phosphate buffer at 4°C, ionic strength 0.1M, pH7.0, centrifuge at 4000rpm for 10min, and the supernatant is TMV-CP.

[0069] According to literature reports, using A 282 Absorption calculation TMV-CP concentra...

preparation example 2

[0071] The DNA sequence of the mutant T153C was obtained by megaprimer site-directed mutagenesis; the DNA sequence and the vector pET21b were constructed as a recombinant plasmid, expressed in Escherichia coli BL21, and affinity purified by a 6×His tag (Ni-NTA column , purchased from Qiagen), was concentrated with a protein concentrator tube (Amicon) to obtain the mutant T153C protein at a concentration of 1.2 μg / uL.

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Abstract

The invention provides a preparation method of a nanobiomaterial and is characterized in that the method comprises a step of selecting nucleic acid with required length and assembling the nucleic acid and a protein for incubation so as to obtain the nanobiomaterial. The nucleic acid can start its assembly with the protein. The assembly incubation condition makes the nucleic acid to be wrapped by the protein such that nuclease can not contact with the nucleic acid through the protein which wraps the nucleic acid. The length of the nucleic acid and granularity of the nanobiomaterial form a linear relation. The method for selecting the nucleic acid with the required length is to determine the required nucleic acid length according to the granularity of the required nanobiomaterial. The invention also provides the nanobiomaterial prepared by the above method and a nucleic acid compound. According to the method provided by the invention, the granularity of the nanobiomaterial is accurately controlled through accurate control of the nucleic acid length. The nanobiomaterial and the nucleic acid compound provided by the invention has good resistance to nuclease.

Description

technical field [0001] The invention relates to a bionano material, a preparation method thereof, and a nucleic acid complex. Background technique [0002] Nanomaterials have very broad applications because of their special properties such as force, heat, sound, light, and electromagnetism. Compared with traditional crystalline materials, nanomaterials have properties such as high strength, high diffusivity, high plasticity, low density, low elastic modulus, high electrical resistance, high specific heat, high thermal expansion coefficient, and low thermal conductivity. These special properties make nanomaterials widely used in high mechanical performance environments, photothermal absorption, nonlinear optics, magnetic recording, special conductors, molecular sieves, ultramicro composite materials, catalysts, heat exchange materials, sensitive components, sintering aids, Lubricants and other fields. [0003] In recent years, various nanomaterials have been widely used in ...

Claims

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Application Information

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Patent Type & AuthorityApplications(China)
IPC IPC(8): A61K48/00A61K47/42
Inventor席真高爽吕琳张偌瑜
OwnerNANKAI UNIV