Kit for detecting mutation condition of rifampicin drug-resistant gene of mycobacterium tuberculosis

Abstract

The invention discloses a kit for detecting mycobacterium tuberculosis rifampicin drug resistance gene mutation, which comprises a first reaction tube, a second reaction tube and a third reaction tube which react under the same QRT-PCR (Quantitative Reverse Transcription-Polymerase Chain Reaction) reaction program, a forward detection primer, a reverse detection primer, an internal reference forward primer, an internal reference reverse primer and an internal reference probe are arranged in each of the first reaction tube, the second reaction tube and the third reaction tube. Based on an MGB probe method and a molecular beacon method derived from a fluorescent quantitative polymerase chain reaction (QRT-PCR) type, in the same reaction system, different probes or molecular beacons are used, three reaction tubes are used for detecting a plurality of point mutation sites in the drug-resistant gene, and according to the Ct value and delta Ct value represented by each point mutation in the system, the point mutation sites in the drug-resistant gene are detected. And determining the point mutation type of the drug-resistant gene in the sample.

Description

technical field [0001] The invention belongs to the technical field of molecular biology, and in particular relates to a kit for detecting the mutation of the rifampicin resistance gene of mycobacterium tuberculosis. Background technique [0002] Mycobacterium tuberculosis is a common pathogen that causes tuberculosis. Tuberculosis is the main pathogenesis mode, and other organs of the body can also be invaded. According to WHO estimates, in 2018, the estimated number of rifampicin-resistant tuberculosis patients worldwide was about 560,000, of which multidrug-resistant tuberculosis accounted for about 82%. Among the 30 countries with a high burden of tuberculosis in the world, India has the largest number of rifampicin-resistant tuberculosis patients (135,000, accounting for 24% of the global total). The number of rifampicin-resistant tuberculosis patients in China calculated based on the estimated number of tuberculosis cases is 73,000 (accounting for 13% of the global t...

AI Technical Summary

Problems solved by technology

And the rpoB gene rpoB of this RNAP and rifampicin binding region subunit portion is prone to mutation under certain circumstances, thus causing the subunit to be difficult to effectively combine with the rifampicin molecule, causing rifampicin to have the mutated gene. Germs lose their effectiveness

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