Method for rapidly detecting thyroxine T4 based on fluorescence resonance transfer
A thyroxine, fluorescence resonance technology, applied in fluorescence/phosphorescence, measurement device, material analysis by optical means, etc., to achieve the effect of improving detection limit, high sensitivity and good selectivity
Pending Publication Date: 2022-06-24
武汉海关技术中心
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Many studies have pointed out that the interference effect of known TDCs only accounts for a small part of the total interference toxicity in the environment or biological samples, and there are a large number of undiscovered interference substances
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Embodiment 1
[0041] The present embodiment provides a method for rapidly detecting thyroxine T4 based on fluorescence resonance transfer, comprising the following steps:
[0042] Step 1: Synthesize carbon quantum dots with amino groups;
[0043] Step 2: Synthesize silica with surface-modified carboxyl groups;
[0044] Step 3: mixing carbon quantum dots with carboxylated silica to carry out amidation reaction;
[0045] Step 4: Make SiO 2 -CD-NH 3 bromination reaction;
[0046] Step 5: Take SiO 2 -CD-Br as the substrate, adding template, monomer, cross-linking agent, catalyst to carry out atom transfer radical reaction;
[0047] Step 6: The addition of the target substance thyroxine T4 makes the fluorescence in the system attenuate.
[0048] Each of the above steps will be described in detail below.
[0049] Step (1), synthesizing carbon quantum dots with amino groups
[0050] Dissolve 300 mg of chitosan in an acetic acid aqueous solution (1:100, V / V) containing 1 mM silver nitrate, ...
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The invention discloses a method for detecting thyroxine T4 based on fluorescence resonance energy transfer. The method comprises the following steps: respectively synthesizing carbon quantum dots with amino groups on the surfaces and silicon dioxide with carboxyl modified on the surfaces, modifying the carboxylated silicon dioxide with the aminated carbon quantum dots through amidation reaction, reacting with dibromoisobutyryl bromide, and introducing bromide ions as an initiator to participate in the following atom transfer radical reaction, thereby obtaining the carbon quantum dot modified silicon dioxide. And finally synthesizing silicon dioxide surface molecular imprinting. A fluorescence resonance energy transfer system is constructed by taking a fluorescence monomer in an atom transfer radical polymerization reaction as an energy donor and carbon quantum dots as an energy receptor, and fluorescence in the system is attenuated when the fluorescence resonance energy transfer system is used, so that a high-sensitivity detection method of thyroxine T4 is established. In addition, the applicant notices the structural similarity of thyroxine and thyroxine interferent, and applies the thyroxine and thyroxine interferent to the detection of thyroxine interferent.
Description
technical field [0001] The invention belongs to the field of environmental monitoring, and more particularly, relates to a method for detecting thyroxine. Background technique [0002] Thyroid hormone is a hormone secreted by the thyroid that acts on almost all cells in the human body. The growth-promoting effect of thyroid hormones is most pronounced in infancy, with the greatest impact during the first five months of life. It mainly promotes the growth and development of bones, brain and reproductive organs. Without thyroid hormone, GH from the pituitary gland cannot function. Furthermore, when thyroid hormones are deficient, the pituitary gland produces and secretes GH less. So congenital or juvenile lack of thyroid hormones, causing cretinism. Cretinism patients have stagnant bone growth and short stature, and the length ratio of the upper and lower bodies is abnormal, and the upper body accounts for more than normal people. In addition, due to the growth disorder o...
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Login to View More IPC IPC(8): G01N21/64
CPCG01N21/6428
Inventor 赵晓亚荆涛王晗尚吟竹王惠王鹏郑茜玥
Owner 武汉海关技术中心