Preparation method and application of mouse hypertension fundus lesion model caused by continuous angiotensin II perfusion

Abstract

The invention discloses a preparation method and application of a hypertensive retinopathy mouse animal model, and belongs to the technical field of biomedicine. According to the method, an implantable micro-pump filled with perfusate is subcutaneously embedded into a mouse, the perfusate is continuously pumped into the body of the mouse, the perfusate contains angiotensin II, the pumping dose of the angiotensin II is 2500-3500 ng / kg / min, and the pumping time is 3-4 weeks. A mouse is used as a model object, and an implanted trace pump is used for continuously pumping angiotensin II to improve systolic pressure of the mouse and increase retinal inflammation and thickness change so as to cause hypertensive retinopathy. The time for constructing the model is short and is only 3 weeks, the constructed mouse hypertensive retinopathy model has central retina thickening, blood vessel arteriovenous proportion change and morphological change, retina electrophysiological change, oxidative stress increase and inflammatory cytokine up-regulation, and basic pathological characteristics of human hypertensive retinopathy are simulated.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a preparation method and application of a mouse hypertensive fundus lesion model caused by continuous angiotensin II perfusion. Background technique [0002] Systemic hypertension is an important risk factor affecting the structure and function of the eye, and the increase of arterial blood pressure is the main reason for the pathophysiological changes and clinical symptoms of the retina of hypertension. Hypertensive retinopathy is present in 3% to 14% of adults over the age of 40. The main clinical manifestations of hypertensive retinopathy include retinal arterial thinning and reflection enhancement, arteriovenous ratio reduction, arteriovenous cross compression sign, patchy hemorrhages along the blood vessels, cotton wool spots, optic disc edema and arteriosclerosis in severe cases. a complication. Its pathophysiological basis is the decrease in the number of...

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Problems solved by technology

However, these models still have limitations: ① animal models are concentrated in large animals such as rats and monkeys, and have high requirements for research sites and research funds; It takes at least 15 months to construct; ③The operation of animal models is relatively difficult, such as the suprarenal aortic coarctation model has high technical difficulty, and corresponding operating rooms need to be equipped to meet the aseptic requirements, so as to reduce infection, etc. The proportion of factors that fail to model

[0005] At present, there is no case of establishing a mouse model for hypertensive retinopathy

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