Application of chondroitin sulfatase in preparation of medicine for treating pain

A chondroitin sulfate enzyme and drug technology, applied in the direction of drug combination, drug delivery, enzymes, etc., can solve the problem of no chondroitin sulfate enzyme

Pending Publication Date: 2022-06-28
YANAN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no research on the pain treatment and corresponding mechanism of chondroitin sulfate enzyme in this field.

Method used

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  • Application of chondroitin sulfatase in preparation of medicine for treating pain
  • Application of chondroitin sulfatase in preparation of medicine for treating pain
  • Application of chondroitin sulfatase in preparation of medicine for treating pain

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1: Chondroitinase ABC relieves formalin-induced spontaneous pain-related behaviors in rats

[0031] Put a stand with a height of 75cm on the table covered with a glass mirror, and place a 20×20×30cm on the shelf 3 A transparent plexiglass box was used to observe the reaction of the rat's foot after administration from the mirror on the table. Before drug injection, the rats were placed in the box 30 minutes in advance to adapt to the experimental environment. After the sole injection of formalin (2.5%, 50 μl), the animals were put back into the detection box, and spontaneous pain behaviors were continuously observed. Nociceptive spontaneous pain response was evaluated by the number of spontaneous foot lifts and foot licking time every 5 minutes.

[0032] Chondroitinase ABC was administered by intrathecal injection of 10 μl of the drug at a concentration of 0.01 U / μl in rats 30 minutes before formalin injection. The effect of Chondroitinase ABC on the behavior...

Embodiment 2

[0034] Example 2: Chondroitinase ABC relieves the behaviors related to spontaneous pain in rats induced by scorpion toxin BmK I

[0035]Put a stand with a height of 75cm on the table covered with a glass mirror, and place a 20×20×30cm on the shelf 3 A transparent plexiglass box was used to observe the reaction of the rat's foot after administration from the mirror on the table. Before drug injection, the rats were placed in the box 30 minutes in advance to adapt to the experimental environment. After the sole injection of scorpion toxin BmK I (10 μg / 50 μl), the animals were put back into the detection box, and the spontaneous pain behavior was continuously observed. The nociceptive spontaneous pain response was evaluated by the number of spontaneous foot lifts per 5 minutes and the number of grand attacks.

[0036] Chondroitinase ABC was administered by intrathecal injection of 10 μl of the drug at a concentration of 0.01 U / μl in rats 30 minutes before the injection of the s...

Embodiment 3

[0038] Example 3: Chondroitinase ABC relieves bilateral mechanical hyperalgesia in rats induced by scorpion toxin BmK I

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PUM

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Abstract

The invention discloses an application of chondrosulphatase in preparation of a medicine for treating pain. By adopting formalin and carrageenan painful animal models and simulating a BmK I mirror image pain model constructed by natural scorpion jellyfish, clinical common symptoms of spontaneous pain, heat pain sensitivity and mechanical pain sensitivity are simulated, so that the chondroitinase ABC has an inhibition effect on the spontaneous pain, the heat pain sensitivity and the mechanical pain sensitivity, and can be used as a new drug target for treating pain.

Description

technical field [0001] The invention belongs to the technical field of application of chondroitinase sulfate, in particular to the application of chondroitinase sulfate in the preparation of medicines for treating pain. Background technique [0002] Pain is one of the biomedical problems that seriously plague human health. Pain is a common feature of most diseases, and is an unpleasant feeling that is common to all humans but varies greatly among individuals. Divided into physiological pain and pathological pain, the former provides early warning signals for the body to be threatened and is an indispensable protective mechanism for the body; the latter has various inducing factors, such as body tissue damage, inflammation, emotional out-of-control, motor sensory disorders, genetics Mutations, tumors, etc. can change the individual's self-protection and internal adaptation plasticity in pain perception, transmission, and integration. The persistence, chronicity, and individu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/51A61P25/00
CPCA61K38/51A61P25/00A61K9/0019C12Y402/0202C12Y402/02021
Inventor 姜鸣白占涛魏倩杨亮杨清湖刘霞
Owner YANAN UNIV
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