Expression factor mutated baculovirus expression vector system

An expression vector and baculovirus technology, applied in the field of baculovirus expression vector system, can solve the problem of low yield of foreign protein and achieve the effect of improving expression

Inactive Publication Date: 2022-07-29
陕西杆粒生物科技有限公司
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  • Summary
  • Abstract
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  • Claims
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AI Technical Summary

Benefits of technology

The inventors discovered that adding certain specific groups at various positions on an enzyme called LFSR (Leuconase Shift Resistance) could increase its ability to control viruses more effectively than other methods like tethering or inserting foreign DNA into their genome. This led them to discover how strong promoter activity may be involved in controlling this process.

Problems solved by technology

Technologies described in this patents involve studying how certain types of microorganisms like Bacteria or Actinosynchema have ability to produce substances called lanthanoids during their growth process. However, there may be some drawbacks associated with current methods involving introducing nucleic acids into living organism's cellular environment.

Method used

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  • Expression factor mutated baculovirus expression vector system
  • Expression factor mutated baculovirus expression vector system

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Embodiment Construction

[0021] In order to make the objectives, technical solutions and advantages of the present invention clearer, the present invention will be further described in detail below with reference to the embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention, but not to limit the present invention.

[0022] The following is an example of the LEF-10 truncated mutant expression cassette located on the transfer vector plasmid (attached below). figure 1 A), the application principle of the present invention is described in detail.

[0023] 1. Clone the GFP reporter gene on the transfer vector plasmid.

[0024] Amplify the eGFP gene with the primers tataccatggtgagcaagggcgagga and ggtgctcgagcttgtacagctcgtccatgc with restriction endonuclease sites, and then clone the eGFP gene into the pTriEx1.1 transfer vector plasmid between the NcoI and XhoI sites to obtain the pTriEx-GFP plasmid. .

[0025] 2. Clone the LEF-10 ...

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Abstract

The invention discloses a baculovirus expression vector system with mutated expression factors. The vector comprises an expression cassette of an LEF-10 carboxyl terminal truncated mutant overexpressed by a heterologous promoter. The overexpression of the LEF-10 carboxyl terminal truncated mutant can significantly improve the expression quantity of exogenous genes. The improvement of the expression yield can reduce the production cost of enterprises. The baculovirus expression vector can be used in the industrial field of biological products.

Description

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Claims

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Application Information

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Owner 陕西杆粒生物科技有限公司
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