Expression factor mutated baculovirus expression vector system

Abstract

The invention discloses a baculovirus expression vector system with mutated expression factors. The vector comprises an expression cassette of an LEF-10 carboxyl terminal truncated mutant overexpressed by a heterologous promoter. The overexpression of the LEF-10 carboxyl terminal truncated mutant can significantly improve the expression quantity of exogenous genes. The improvement of the expression yield can reduce the production cost of enterprises. The baculovirus expression vector can be used in the industrial field of biological products.

Description

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AI Technical Summary

Benefits of technology

The inventors discovered that adding certain specific groups at various positions on an enzyme called LFSR (Leuconase Shift Resistance) could increase its ability to control viruses more effectively than other methods like tethering or inserting foreign DNA into their genome. This led them to discover how strong promoter activity may be involved in controlling this process.

Problems solved by technology

Technologies described in this patents involve studying how certain types of microorganisms like Bacteria or Actinosynchema have ability to produce substances called lanthanoids during their growth process. However, there may be some drawbacks associated with current methods involving introducing nucleic acids into living organism's cellular environment.

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