Production method of recombination fibroblast growth factor-8b and application thereof

A technology of fibroblasts and growth factors, applied in the field of genetic engineering, to achieve the effect of fast and convenient acquisition and promotion of cell proliferation

Inactive Publication Date: 2016-03-02
WENZHOU MEDICAL UNIV +1
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] One of the purposes of the present invention is to provide a production method for recombinant fibroblast growth factor-8b protein, to...

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Production method of recombination fibroblast growth factor-8b and application thereof
  • Production method of recombination fibroblast growth factor-8b and application thereof
  • Production method of recombination fibroblast growth factor-8b and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0046] In accordance with the present invention, conventional molecular biology, microbiology, cytology and DNA recombinant techniques within the skill of the art may be employed. Definitions of terms where they appear in this document are as follows.

[0047] "DNA molecule" refers to the polymeric form of deoxyribonucleotides (thymine, cytosine, adenine or guanine), which are the main constituents of chromosomes and the material that makes up genes. This term refers only to the primary and secondary structure of the molecule, without restricting it to any specific tertiary form. The term includes double-stranded DNA found especially in linear DNA molecules, viruses, chromosomes, plasmids. The structure discussed here is conventionally given only the sequence of the sense strand of DNA in the 5' to 3' direction.

[0048] A "vector" refers to a nucleic acid molecule capable of transporting another nucleic acid to which it has been ligated. One type of vector is a "plasmid," w...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a production method of a recombination fibroblast growth factor-8b protein. The method comprises the following steps that 1, an insect baculovirus expression vector system is utilized for expressing a fibroblast growth factor-8b protein in an insect cell, wherein the recombination baculovirus Bacmid-FGF8b is obtained, genes of the FGF8b are fibroblast growth factor-8b genes, and the base sequence of the FGF8b is shown in the SEQ ID No:1; the Bacmid-FGF8b recombination baculovirus of the certain MOI obtained in the first step is used for infecting insect cells, and vibration and suspension are performed at the temperature of 25-29 DEG C for culture for 36-120 h after infection is performed to obtain a cell sap; 2, the fibroblast growth factor-8b protein is obtained by purifying the cell sap obtained in the first step. The invention further discloses application of the fibroblast growth factor-8b protein for treating the Parkinson disease.

Description

technical field [0001] The invention relates to the field of genetic engineering, in particular to a production method and application of recombinant fibroblast growth factor-8b. Background technique [0002] Fibroblast growth factor-8 (fibroblast growth factor 8, FGF-8) is a member of the fibroblast growth factor family, and has 30% to 40% homology with known family members. FGF8 was first extracted from the androgen-dependent mouse breast cancer cell line SC-3. The human FGF8 gene is located in the chromosome 10q24 segment, has 6 exons, and is composed of 233 amino acids. The mature FGF8 is a polypeptide composed of 194 amino acid residues (molecular weight is about 23kDa) by removing the N-terminal 39 amino acid signal peptide. It is a basic protein with an isoelectric point of about 10.5. Human FGF8 has four subtypes, a, b, e, and f, and FGF8b is its dominant expression type, and its binding ability to FGF receptors is the strongest. Extensive literature reports that ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N15/866C07K14/50A61P25/16
Inventor 姜潮马吉胜田海山刘敏陈娜子
Owner WENZHOU MEDICAL UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap