Colloidal gold chromatography test strip for rapidly detecting lymph node metastasis of papillary thyroid carcinoma and preparation method of colloidal gold chromatography test strip

Abstract

The invention discloses a colloidal gold chromatography test strip for rapidly detecting thyroid papillary carcinoma lymph node metastasis and a preparation method of the colloidal gold chromatography test strip. A sample pad, a combination pad, a nitrocellulose membrane and an absorption pad are sequentially overlapped at one end of a bottom plate from left to right; the binding pad is a polyester film sprayed with a Cyfra21-1/Braf V600E capture antibody-colloidal gold compound, the nitrocellulose film is coated with a detection line T and a quality control line C, an antibody in the detection line T is a Cyfra21-1/Braf V600E monoclonal antibody, and the quality control line C is goat anti-mouse IgG. The occurrence rate of false negative is reduced by improving the tissue utilization degree, the detection speed is increased through a chromatography test strip method, finally the purposes of reducing the missed diagnosis rate, relieving the disease burden of a patient and improving the working efficiency of thyroid surgery are achieved, and important clinical significance is achieved.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a colloidal gold chromatography test strip for rapidly detecting lymph node metastasis of papillary thyroid carcinoma and a preparation method thereof. Background technique [0002] In 2020, there will be 586,202 new cases of thyroid cancer in the world, ranking ninth in new cases of cancer in the world. Thyroid cancer is the most common malignant tumor of the head and neck and endocrine system. Except for a clear relationship with exposure to radioactive substances, the pathogenesis of most thyroid cancers is unclear. The best treatment method is early detection, early diagnosis and early treatment, which is the key to improving the clinical diagnosis and treatment of thyroid cancer. Among them, 95% of thyroid cancer is papillary thyroid cancer, and 60%-80% of papillary thyroid cancer patients are accompanied by cervical lymph node enlargement when they first visit a doctor....

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Problems solved by technology

[0004] The BRAF gene is located on chromosome 7q34 and is involved in the signal transduction of the RasRAF-MAPK and extracellular signal-regulated kinase-MAPK pathways. The mutation of codon 600 is common, leading to the continuous activation of BRAF kinase, which is the high expression of the downstream transduction pathway of MAPK, resulting in excessive cell proliferation. deterioration

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