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Newcastle disease virus HN and chick anemia virus VP3 gene joined antineoplastic biologic preparation

A technology for Newcastle disease virus and tumor, applied in the field of eukaryotic recombinant expression vector, can solve the problem of no combined anti-tumor of NDVHN and VP3, and achieve the effects of convenient storage and transportation, reliability and safety, and simple production.

Inactive Publication Date: 2004-07-07
MILITARY VETERINARY INST MILITARY SUPPIES PLA
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  • Abstract
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Problems solved by technology

At present, there is no report on the use of NDV HN nucleic acid vaccine against tumors at home and abroad, and there is no record of NDV HN and VP3 combined anti-tumor

Method used

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  • Newcastle disease virus HN and chick anemia virus VP3 gene joined antineoplastic biologic preparation
  • Newcastle disease virus HN and chick anemia virus VP3 gene joined antineoplastic biologic preparation
  • Newcastle disease virus HN and chick anemia virus VP3 gene joined antineoplastic biologic preparation

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Embodiment approach

[0022] Describe the embodiment of the present invention below by the construction of expression vector and application method

[0023] (1) Basic operation method of DNA recombination

[0024] 1. Preparation and transformation of Escherichia coli competent cells

[0025] Escherichia coli competent cells were prepared by the calcium chloride preparation method. For specific methods, refer to the second edition of "Molecular Cloning Experiment Guide" translated by Jin Dongyan, Li Mengfeng, etc., Science Press (1998), pages 55-56. Just use 0.75mmol / L Tris-HCl instead of water to prepare 0.1mol / L CaCl 2 . For the transformation method of competent cells, refer to the above-mentioned references.

[0026] 2. Mass Preparation of Plasmids

[0027] Referring to the second edition of "Molecular Cloning Experiment Guide" translated by Jin Dongyan, Li Mengfeng, etc., Science Press (1998), pages 26-28, it was prepared by alkaline lysis and purified by PEG precipitation. The specific op...

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Abstract

The present invention discloses 3 antineoplastic nucleovaccines able to express newcastle disease virus HN gene and chick anemia virus VP3 gene: pIRHNVP3, pVHN and pVVP3. After transfection of eukaryotic cell, the pIRHNVP3 can express HN protein and VP3 protein at the same time. After mixing and transfection of pVHN and pVVP3, HN protein and VP3 protein can be obtained. Said nucleovaccines and specific proteins can be used to prevent and treat tumor of people and animals.

Description

technical field [0001] The invention provides three eukaryotic recombinant expression vectors of Newcastle disease virus (NDV) HN and chicken anemia virus apoptin gene (VP3), belonging to the field of biotechnology. Background technique: [0002] The eukaryotic recombination system is different from any traditional vaccine or genetic engineering vaccine. It inoculates a small amount of host bacteria carrying gene vaccine expression plasmids into ordinary bacterial growth medium to obtain a large number of eukaryotic recombination plasmids with unlimited replication. The attenuated strain of Newcastle disease virus (NDV) can selectively kill a variety of tumor cells in humans and animals, but has no effect on normal human cells. Similar to the attenuated strain of NDV, the apoptin gene (VP3) of chicken anemia virus (CAV) has a wide range of functions in inducing tumor cell apoptosis. The anti-tumor gene of NDV and CAV is inserted into the eukaryotic expression vector to obta...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61P35/00C12N15/79C12N15/86
Inventor 金宁一龚伟薛立娟丁壮葛涛金扩世郭志儒王宏伟李萍
Owner MILITARY VETERINARY INST MILITARY SUPPIES PLA
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