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Prawn feed immune polysaccharide
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A technology of immune polysaccharide and prawn feed, applied in animal feed, animal feed, application, etc., can solve the problems of high production cost, organic solvent method and alkali-acid method are not environmentally friendly, easy to cause cancer, etc., to avoid large product loss, Conducive to degradation and extraction, cost-saving effect
Inactive Publication Date: 2004-12-29
广东省农业科学院畜牧研究所
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Problems solved by technology
The β-1,3-glucan obtained by the above method is developed into prawn feed immune polysaccharide feed additive, which mainly has the following defects: (1) high investment, requiring a large amount of special equipment; (2) high production cost: organic solvent extraction method The cost of the organic solvent used in the method is high, it takes a lot of energy to wash the acid and alkali in the alkali-acid method, and it needs to react at high temperature, the energy consumption is large, and the enzymatic extraction needs to add a large amount of enzyme preparation with higher price; (3) organic solvent The method and the alkali-acid method are not environmentally friendly, will also produce toxicity, and have a certain degree of corrosion; (4) It is known that the molecular weight of β-1,3-glucan with the best immune effect can be 100,000 Daltons, However, the use of dextranase in the existing enzymatic extraction method cannot ensure that the product has the molecular weight to exert the best performance
[0004] At present, the quality and scientific and technological content of prawn feed are still lagging behind. Disease control is still the primary problem in the process of prawn farming. There are also common problems such as the abuse of antibiotics, hormones, and even the use of some carcinogenic and mutagenic chemical compounds for the purpose of curing diseases and promoting growth. other real problems
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[0022] The preparation process of the prawn feed immune polysaccharide in the present embodiment is as follows: figure 1 , including the following steps in order:
[0023] (a) High-pressure homogeneous crushing: at 5m 3 Add 2.5 tons of yeast mud in the blending tank, wherein the solids are 15% (weight), and the high-pressure homogenizer is pumped into the machine for processing by the delivery pump carried by the high-pressure homogenizer.
[0024] (b) Enzyme hydrolysis, plate and frame filter press: pump the broken yeast cells obtained in step (a) into the reaction tank, add 12 kg of salt, 10 kg of food grade ethanol, and adjust the pH from 6.0 to 6.0 with sodium hydroxide or hydrochloric acid 6.5, open the steam heating valve, raise the temperature of the reaction solution from room temperature to 55°C, pay attention to controlling the heating rate, and complete the temperature rise in about 60 minutes; add papain, the dosage is 60IU / g substrate, and stir the enzymolysis re...
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Abstract
An immunopolyose feed for prawn is prepared from the discarded yeast mud generated during brewing beer through high-pressure (200-600 MPa) shock-pulverizing for homogenizing 1-4 times, adding table salt and edible alcohol, enzymolyzing at 45-60 deg.C, pressure-filtering, superfine breaking of filtered dregs in liquid, pressure filtering again, acidifying and keeping temp. in an intermediate tank, and spray-drying. Its advnatages are low cost and high effect on improving the resistance of prawn to disease.
Description
technical field [0001] The invention relates to a prawn feed immune polysaccharide, in particular to a prawn feed immune polysaccharide containing β-1,3-glucan. Background technique [0002] The yeast cell wall is mainly composed of the following components: alkali-insoluble β-1,3-glucan, alkali-soluble β-1,3-glucan (glucan branch chain with β-1,6 bonds) , Acid-soluble β-1,6-glucan (glucan branched chain with β-1,3 bonds), alkali-soluble mannan combined with protein, etc. Among them, β-1,3-glucan has an immunostimulatory effect, and its sources include: Saccharomyces cerevisiae, Kluyveromyces fragilis, Candida, fungi, unicellular algae, etc. Alkali-insoluble β-1,3-glucan has the strongest effect. In addition to human beings, aquatic animal immunology studies have confirmed that it is effective for certain artificially farmed fish (such as: Atlantic salmon, catfish, rainbow trout and giant flounder) and Prawns (Penaeus monodon, Penaeus vannamei, and Penaeus sinensis) have s...
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