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An optical circulation amplification type biological chip detecting process

A biochip, light cycle technology, applied in the field of biological analysis, achieves the effect of less reagents, convenient operation, and solving the high cost of detection

Inactive Publication Date: 2005-10-12
谢斌
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AI Technical Summary

Problems solved by technology

There are no reports and applications of bioluminescence, an exquisite mechanism in living organisms, applied to biochips.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0017] Screening of Hepatitis Antibody Drugs:

[0018] 1. Separation and purification of various phages that display various possible hepatitis antibodies through phage display technology, and then fix them in each reaction chamber on the chip by acetaldehyde method, and number each antibody.

[0019] 2. Use reduced nicotinamide adenine dinucleotide (NADH) or reduced nicotinamide adenine dinucleotide phosphate (NADPH) and flavin mononucleotide (FMN) specific oxidoreductase (NADH-FMNOxidereductase) Label the inactivated hepatitis virus.

[0020] 3. Add 0.005 μl of enzyme-labeled inactivated hepatitis C virus into each reaction chamber with a chip spotter, and incubate at 37° C. for 30 minutes.

[0021] 4. First wash the non-specifically bound enzyme label with sodium phosphate buffer solution to inactivate hepatitis virus and other non-specific binding substances.

[0022] 5. Add reduced nicotinamide adenine dinucleotide (NADH), flavin mononucleotide (FMN) and phosphate solut...

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Abstract

The invention discloses an optical circulation enlarged type biological chip detecting method, wherein the enzyme recirculation and bioluminescence are combined, for realizing the FMNH#-[2] accumulation through the circulation on the electrodes by the NADH / NAD#+[+] oxidation-reduction, the high level accumulation FMNH#-[2] reacts with other light-emitting bottom luciferase, long chain aldehydes and oxygen, giving off fluorescent light having wavelength of 490nm. The invention realizes high sensibility, low cost and simplicity of operation, thus is suitable for each types of biological sample analysis.

Description

technical field [0001] The invention relates to biological analysis technology, and more specifically relates to a detection method of a photocycle-amplified biological chip, which is suitable for the analysis of various biological samples on the biological chip. Background technique [0002] At present, the detection methods of biochips are mainly based on radioactive isotopes and optical detection, but due to the pollution and hazards of isotopes, people try to avoid the use of it, so fluorescence and chemiluminescence have become the main detection methods of biochips. However, because the extremely low concentration of the biological sample to be tested limits the concentration of the label, only a very weak optical signal can be detected, which requires the use of a very sensitive detection device, such as: luminescent coupling device (cooled CCD), to To capture this signal, the price of these devices is still very high at present, obviously this has...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/25G01N33/50G01N33/576
Inventor 谢斌梁振普谢卫红丹尼尔森・本特
Owner 谢斌
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