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Technique for improving activity of Job's-tears seed

A process method, coix seed technology, applied in the field of microbial transformation technology to process traditional Chinese medicine

Inactive Publication Date: 2007-02-07
YUNNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] There is no effective method to improve and increase the medicinal active ingredients of coix seed by adopting a certain process and then extracting them.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Example 1: Take 10 kg of coix seed → crush until it can pass through a 40-mesh sieve, put it in a 50-liter fermentation container → add 500 g of Rhizopus oryzae wheat bran culture → add 1.2 times the amount of water → stir evenly → 80 ℃ conversion treatment for 4-12 hours → the product of the present invention.

[0017] The product of the present invention is an intermediate product, which is soaked by directly adding 6 times the amount of 95% ethanol for three times by immersion method, concentrated and dried under reduced pressure to obtain 600g of jelly-like extract, and after the jelly-like thing is extracted with acetone, it can be obtained 200g of oily matter containing anticancer components is more than 30% more than the total amount of oily matter extracted by the same method from coicis without this method. The product was tested for anti-cancer activity by MTT method, and it was effective against cancer cell lines such as A549, BEL-7402, B16, SPC-A1, K562, and...

Embodiment 2

[0018] Embodiment 2: prepare 3%--10% aqueous solution of coix seed powder→sterilize→inoculate pure rhizopus oryzae, shake bottle fermentation at 28°C for 3-7 days→contain the culture of rhizopus in a weight ratio of 1% Add to fully moist coix powder → stir evenly → convert at 60°C for 12-25 hours → product of the present invention. Subsequent processing is the same as in Example 1.

Embodiment 3

[0019] Example 3: Prepare potato liquid culture medium → sterilize → inoculate pure rhizopus oryzae, shake flask fermentation at 28°C for 3-7 days → add 2-10 times the amount of ammonium sulfate in the medium, filter the precipitate, and obtain roots Mold crude enzyme preparation → adding 0.05% rhizopus enzyme in the coix seed powder by weight percentage to the coix seed powder → adding 1 times the amount of water → stirring evenly → converting at 60°C for 6-16 hours → the product of the present invention.

[0020] The product of the present invention is an intermediate product, which adopts the dipping method to directly add 20% ethanol of 6 times the amount for three times to soak, filter to obtain the filtrate, and when the filtrate is concentrated to 5-10 liters, add 1.5 times the amount of 95% ethanol, Filter out the precipitate → recover the alcohol in the filtrate, and continue to evaporate to dryness to obtain 500 grams of yellow-brown substance. The yield of this subst...

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Abstract

The invention relates to a method for treating Chinese medicinal herbs by means of microorganism transformation technology, which comprises comminuting the Job's-tears seed, placing into container, charging water so as to make it completely moistening, charging cultured rhizopus culture or enzymes produced by the rhizopus bacterium into the Job's-tears seed powder, stirring homogeneously, fermenting under the temperature of 40-100 deg. C for 4-30 hours.

Description

technical field [0001] The invention relates to a method for treating traditional Chinese medicines with microbial transformation technology. technical background [0002] Coix seed is a kind of traditional Chinese medicine with both medicine and food. It has many functions such as anti-cancer, anti-virus, and immune regulation. It can be developed into various drugs, health food, beauty cosmetics, etc. In 1957, Zongchun Zhongshan, Japan found that the ethanol extract of coix seed had anti-cancer effects. In 1984, Mitsuhiro Numata researched that the anti-cancer components were palmitic acid, stearic acid, oleic acid, and linoleic acid. my country's Liu Hezhong invented a method of extracting anti-cancer substance neutral fat from coix seed by Soxhlet extraction method (91106269.6), and also applied for the use of coix seed extract in the preparation and treatment of erythema keratosis, scaly hair follicle angle Li Dapeng (Application No. 95115453.2) applied for the new medi...

Claims

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Application Information

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IPC IPC(8): C12P1/00A61K36/899A61K131/00A61P31/12A61P35/00A61P37/02
Inventor 王兴红
Owner YUNNAN UNIV
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