Laundry detergent and/or fabric care composition comprising modified cellulase

A technology of laundry detergent and cellulase, applied in detergent composition, detergent compounding agent, fiber treatment, etc.

Inactive Publication Date: 2001-08-08
THE PROCTER & GAMBLE COMPANY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] However, none of these documents disclose modified enzymes for laundry and/or fabric care applications comprising the catalytically active amino acid sequence of a cellulase that is compatible with a cellulose-containi

Method used

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Examples

Experimental program
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preparation example Construction

[0022] They can be prepared, for example, by transforming into a host cell a DNA construct comprising at least one fragment of the DNA encoding the cellulose-binding domain, with or without a linker, with the DNA encoding the beneficial enzyme The sequences are ligated, and transformed host cells are grown to express the fused gene. A related, but non-limiting class of recombinant products obtained in this regard (hybrid enzymes), commonly referred to in the art as "fusion proteins", can be described by one of the following general formulas:

[0023] A-CBD-MR-X-B

[0024] A-X-MR-CBD-B In the latter formula, CBD is an amino acid sequence comprising at least the cellulose binding domain (CBD) itself.

[0025] MR (middle region; linking region) may be a bond, comprising a linker of 1 to about 100 amino acid residues, especially 2-40 amino acid residues, eg 2-15 amino acid residues. MRs can be primarily, additionally non-amino acid linkers (see below).

[0026] X is an amino ac...

Embodiment 2

[0281]The following granular laundry detergent compositions especially suitable for European washing machine conditions were prepared according to the invention: I II III IV V VI LAS 5.5 7.5 5.0 5.0 6.0 7.0TAS 1.25 1.9 - 0.8 0.4 0.3C24AS / C25AS - 2.2 5.0 5.0 5.0 2.2C25E3S - 0.8 1.0 1.5 3.0 1.0C45E7 3.25 - - - - 3.0TFAA - - 2.0 - - -C25E5 - 5.5 - - - -QAS 0.8 - - - - -QAS1 - 0.7 1.0 0.5 1.0 0.7STPP 19.7 - - - - -Zeolite A - 19.5 25.0 19.5 20.0 17.0 NaSKS-6 / citric acid - 10.6 - 10.6 - - (79:21) Na-SKS-6 - - 9.0 - 10.0 10.0 Carbonate 6.1 21.4 9.0 10.0 10.0 18.0 Bicarbonate - 2.0 7.0 5.0 - 2.0 Silicate 6.8 - - 0.3 0.5 - Citrate - - 4.0 4.0 - - Sulfate 39.8 - - 5.0 - 12.0 Magnesium Sulfate - - 0.1 0.2 0.2 -MA / AA 0.5 1.6 3.0 4.0 1.0 1.0CMC 0.2 0.4 1.0 1.0 0.4 0.4PB4 5.0 12.7 - - - - Percarbonate - - - - 18.0 15.0TAED 0.5 3.1 - - 5.0 -NACA-OBS 1.0 3.5 - - - 2.5DETPMP 0.25 0.2 0.3 0.4 - 0.2HEDP...

Embodiment 3

[0283] The following detergent formulations, particularly suitable for European washing machine conditions, were prepared according to the invention: I II III IV Blown powder LAS 6.0 5.0 11.0 6.0 TAS 2.0 - - 2.0 Zeolite A 24.0 - - 20.0 STPP - 27.0 24.0 - Sulfate 4.0 6.0 13.0 - MA / AA 1.0 4.0 6.0 2.0 Silicate 1.0 7.0 3.0 3.0 CMC 1.0 1.0 0.5 0.6 Brightener 1 0.2 0.2 0.2 0.2 Silicone defoamer 1.0 1.0 1.0 0.3 DETPMP 0.4 0.4 0.2 0.4 Spray on Ⅰ Ⅱ Brightener 0.02 - - 0.02 C45E7 - - - 5.0 C45E2 2.5 2.5 2.0 - C45E3 2.6 2.5 2.0 - Fragrance 0.5 0.3 0.5 0.2

[0284] Silicone defoamer 0.3 0.3 0.3 - Dry additive QEA - - - 1.0 EDDS 0.3 - - - Sulfate 2.0 3.0 5.0 10.0 Carbonate 6.0 13.0 15.0 14.0 Citric acid 2.5 - - 2.0 QAS1 0.5 - - 0.5 Na-SKS -6 10.0 - - - Percarbonate 18.5 - - - PB4 - 18.0 10.0 21.5 TAED 2.0 2.0 - 2.0 NACA-OBS 3.0 2.0 4.0 -CBD-Cellulase 0.0005 0.01 0.005 0.01 Cellulase 0.0004 - - 0.0008 Protease 0.03 0.03 0.03 Lipase 0.008 0.008 0.008 0.004 Amylase 0.003 0.003 0.003 0.006 Br...

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Abstract

The present invention relates to a modified enzyme which comprises a catalytically active amino acid sequence of a cellulolytic enzyme linked to an amino acid sequence comprising a Cellulose Binding Domain (CBD) having a relative binding constant (Kr-a) for binding to amorphous cellulose higher than 2.4l/gcellulose, preferably higher than 3.5l/gcellulose, more preferably higher than 4l/gcellulose, for selective binding and hydrolysis of amorphous cellulose of cotton containing fabrics in a laundry and/or fabric care application. The present invention further relates to laundry detergent and/or fabric care compositions comprising this modified enzyme.

Description

field of invention [0001] The present invention relates to laundry detergent and / or fabric care compositions comprising a modified enzyme comprising a catalytically active amino acid sequence of a cellulase which is compared to a cellulase comprising a relative binding constant (Kr-a ) greater than 2.4 l / g cellulose, preferably greater than 3.5 l / g cellulose, more preferably greater than 4 l / g cellulose amino acid sequence linkages of the cellulose binding domain (CBD). Background of the invention [0002] Detergent compositions today comprise complex mixtures of active ingredients and especially detergent enzymes such as cellulases. [0003] Cellulase activity is a property of a cellulase to attack a cellulosic fiber or substrate, which depends on the cellulase, which can be an endo- or exo-cellulase, and the specific efficacy of each hemicellulase. The cellulose structure can be depolymerized or broken up into smaller and thus more soluble or dispersib...

Claims

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Application Information

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IPC IPC(8): C11D3/386C12N9/42D06M16/00
CPCD06M16/003C12Y302/01004C11D3/38645C12N9/2434C12N9/2437
Inventor J·斯梅茨A·布施A·C·贝克J·-L·P·贝蒂奥尔S·L·波耶尔
Owner THE PROCTER & GAMBLE COMPANY
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