Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Interferon gamma conjugates

A technology of conjugates and interferon, applied in the direction of interferon, cytokines/lymphokines/interferon, drug combinations, etc., can solve problems such as modifications that have not been discussed in detail, and achieve long intervals, effective therapeutic responses, and enhanced efficacy Effect

Inactive Publication Date: 2007-04-11
MAXYGEN HLDG
View PDF59 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

IFNG is mentioned as an example of a member of the growth hormone superfamily, but its modifications are not discussed in detail

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0278] Preparation of pharmaceutical compositions

[0279] According to methods well known to those skilled in the art, the conjugates of the present invention related to purification can be formulated in such a way that each vial contains 50, 100, 200, 300, 400 or 500 micrograms of rhuIFNG or IFNG-N25K conjugates. Compositions for injection, thereby preparing, for example, pharmaceutical compositions for the treatment of interstitial lung disease.

[0280] Identification of surface-exposed amino acid residues

[0281] structure

[0282] The experimental three-dimensional structure of huIFNG determined by X-ray crystallography has been reported by: Ealick et al., Science 252:698-702 (1991), which reported the C-alpha trace of the IFNG homodimer. Walter et al., Nature 376:230-235 (1995) reported the structure of an IFNG homodimer complexed with two molecules of soluble IFNG receptor. The coordinates of the structure have never been made public. Thiel et al., Structure 8: 92...

Embodiment 1

[0326] Design of expression cassettes capable of expressing IFNG in yeast and CHO cells

[0327] The DNA sequence with GenBank accession number X13274 contains the full-length cDNA encoding mature huIFNG without its native signal peptide, modified to facilitate efficient expression in yeast cells. First, a MATa signal peptide was introduced instead of the IFNG signal peptide to facilitate secretion into the yeast medium. Second, the codons of the huIFNG nucleotide sequence were modified by adopting a bias in codon usage towards codons commonly used in yeast. Certain nucleotides in this sequence are then substituted with other nucleotides in order to introduce recognition sites for DNA restriction endonucleases. Primers were designed to allow synthesis of the gene. Primers were assembled into synthetic genes by one-step PCR using the Platinum Pfx-polymerase kit (Life Technologies) and standard three-step PCR cycling parameters. The assembled gene, which has the sequence show...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
molecular weightaaaaaaaaaa
molecular weightaaaaaaaaaa
diameteraaaaaaaaaa
Login to View More

Abstract

A conjugate exhibiting interferon gamma activity and comprising at least one first non-polypeptide moiety covalently linked to an IFG polypeptide, the polypeptide comprising an amino acid sequence that differs from that of a parent IFNG polypeptide in at least one introduced and / or at least one removed amino acid residue comprising an attachment group for the non-polypeptide moiety. The conjugate may be used for treatment of various diseases.

Description

field of invention [0001] The present invention relates to a conjugate with interferon gamma-like activity, a preparation method thereof, a pharmaceutical composition containing the molecule and its use in disease treatment. Background of the invention [0002] Interferon-gamma (IFNG) is a cytokine produced by T-lymphocytes and natural killer cells and exists as a homodimer of two non-covalently bound polypeptide subunits. The mature form of each dimer contains 143 amino acid residues (shown in SEQ ID NO 2), and its precursor form includes a signal sequence of 166 amino acid residues (shown in SEQ ID NO 1). [0003] Each subunit has two potential N-glycosylation sites (Aggarwal et al., Human Cytokine, Blackwell Scientific Press, 1992) at positions 25 and 97. The dimeric form of IFNG has a molecular weight of 34-50 kDa depending on the degree of glycosylation (Farrar et al., Annals of Immunology, 1993, 11:571-611). [0004] Gray et al. (Nature, 2...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A61K47/48C07K14/57A61K38/21A61P37/00A61K38/00
CPCC07K14/57A61K38/00A61P37/00Y02P20/55
Inventor 安妮·D·詹森金·V·安德森克里斯琴·K·汉森
Owner MAXYGEN HLDG
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com