Oriental fir tissue culture quick propagation technology and basic culture medium

A basic medium, the technology of Oriental fir, which is applied in the field of MHJ formula suitable for the basic medium of Oriental fir, can solve the problems that Oriental fir cannot be propagated and promoted in large quantities, there are no seeds for sowing and propagation, and the survival rate of cutting propagation is low.

Inactive Publication Date: 2007-08-01
上海光兆植物速生技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Due to the fact that Oriental fir is only cultivated in a small amount at present, there are no seeds for sowing and reproduction, and the survival rate of cutting propagation is very low, so that Oriental fir cannot be propagated and promoted in large quantities.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] According to formula one, the method of rapid propagation of Oriental fir tissue culture:

[0029] First, determine the formula of the basic medium MHJ suitable for Oriental fir, see formula 1 in Table 2;

[0030] Second, after sterilization of the explants and establishment of sterile seedlings, the Oriental fir test-tube seedlings are rapidly multiplied by tissue culture through the method of stem micro-cutting and bud mass propagation. Among them: a) Micro-cutting: remove the stem top of the test-tube seedling Cut the cuttings and place them on the medium: MHJ+ activated carbon 5g / l, continue high-growth culture, and cut the remaining stems into small pieces of about 0.5cm, and cut them into the (D-1) medium: MHJ+6-BA1.0mg / l+ Activated carbon 5g / l induces axillary buds to germinate and extract new buds. After the new buds are cut, they are grown to a height of 5cm, and then cut into stem segments for micro-cutting propagation. The propagation ratio reaches about 1:5; b) ...

Embodiment 2

[0034] According to the formula 2 in Table 2, the fast propagation method of Oriental fir tissue culture:

[0035] First, determine the formula of the basic medium MHJ suitable for Oriental fir, see formula 2 in Table 2;

[0036] Second, after sterilization of the explants and establishment of sterile seedlings, the Oriental fir test-tube seedlings are rapidly multiplied by the method of micro-cutting of stems and bud mass propagation. Among them: a) Micro-cutting: the top of the stem of the test tube seedling Cut the cuttings and place them on (D-2) medium: MHJ+ activated carbon 3g / l, continue high-growth culture, and cut the remaining stems into small pieces of about 0.5 cm and cut them into (D-1) medium: MHJ+6- BA0.5mg / l+activated carbon 3g / l induces axillary buds to germinate and extract new buds. After the new buds are cut, they are grown to a height of 5cm and then cut into stem segments for micro-cutting propagation. The propagation ratio reaches about 1:5; b ) Bud mass pro...

Embodiment 3

[0040] According to the formula 3 in Table 2, the fast propagation method of Oriental fir tissue culture:

[0041] First, determine the formula of the basic medium MHJ suitable for Oriental fir, see formula 3 in Table 2;

[0042] Second, after sterilization of the explants and establishment of sterile seedlings, the Oriental fir test-tube seedlings are rapidly multiplied by the method of micro-cutting of stems and bud mass propagation. Among them: a) Micro-cutting: the top of the stem of the test-tube seedling Cut the cuttings and place them on the (D-2) medium: MHJ+ activated carbon 1g / l, continue high-growth culture, and cut the remaining stems into small pieces of about 0.5 cm. Cut into the (D-1) medium: MHJ+6- BA0.2mg / l+ activated carbon 1g / l induces axillary buds to germinate and extract new buds. After the new buds are cut, they are grown and cultivated to a height of 5cm, and then cut into stem segments to circulate for micro-cutting propagation, and the propagation ratio r...

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Abstract

Disclosed is the fast tissue culturing and propagating technology for Taxodium mucronatum X Cryptomeria fortunei, wherein the basic cultivation medium of Taxodium mucronatum X Cryptomeria fortunei tissue cultivation comprises a large quantity of elements, ferric salts, organics, microelements and other substance, the fast tissue culturing and propagating method comprises explant sterilization, sterile sprout establishment, test tube sprout breeding, root culturing and transplanting.

Description

Technical field [0001] The invention relates to a method for rapid propagation of oriental fir tissue culture and a related suitable oriental fir basic medium MHJ formula. Background technique [0002] Oriental fir is a new tree species obtained by cross-genus hybridization of Chinese cedar and Mexican cypress in 1963 by Professor Ye Peizhong, a famous Chinese forest tree breeder. After decades of cutting selection and breeding, the embryonic form of a new tree species was formed. After the introduction, Shanghai found that Oriental fir grew well in the coastal area of ​​Shanghai. This rare inter-genus hybrid tree species has the following characteristics: the tree is tall and graceful, inheriting the graceful tree shape of Cryptomeria and Mexican deciduous; the deciduous period is shorter (from mid-January to early March), which is better than Metasequoia. , Pond fir and cypress cedar should be shorter for 1-2 months to make the viewing period longer; the branches are strong, wh...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/04A01H4/00A01G17/00
Inventor 宋学孟苏敏庞瑞华王凌健陈权董举文楼文阜何燕红
Owner 上海光兆植物速生技术有限公司
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