Oriental fir tissue culture quick propagation technology and basic culture medium

A basic culture medium, tissue culture rapid propagation technology, applied in cultivation, horticultural methods, botanical equipment and methods, etc., can solve the problem that Oriental fir cannot be propagated and promoted in large quantities, there are no seeds for sowing and propagation, and the survival rate of cutting propagation is low and other problems to achieve the effect of solving the problem of test tube asexual reproduction

Inactive Publication Date: 2005-07-06
上海光兆植物速生技术有限公司
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  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Due to the fact that Oriental fir is only cultivated in a small amount at present, there are no seeds for sowing and reproduction, and the survival rate of cutting propagation is very low, so that Oriental fir cannot be propagated and promoted in large quantities.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] According to formula 1, the tissue culture and rapid propagation method of Dongfang fir:

[0027] First, determine the formula of the basic medium MHJ suitable for Oriental fir in formula one of Table 2;

[0028] Second, after explant disinfection and the establishment of sterile seedlings, the test-tube seedlings of Oriental fir are rapidly multiplied by tissue culture through stem section micro-cutting and bud mass propagation methods, wherein: a) micro-cutting: put the stem top of the test-tube seedling Cut off the cuttings and place them on (D-2) medium: MHJ+activated carbon 5g / l, continue high-growth culture, and cut the remaining stems into small pieces of about 0.5cm and cut them into (D-1) medium: MHJ+6- BA1.0mg / l+activated carbon 5g / l induces axillary buds to germinate and pull out new shoots. After the new shoots are cut off, they are grown to a height of 5 cm and then cut into stem segments for micro-cutting propagation. The propagation ratio reaches about 1:...

Embodiment 2

[0032] According to the formula 2 in Table 2, the tissue culture and rapid propagation method of Oriental fir:

[0033] First, determine the formula of the basic medium MHJ suitable for Oriental fir, see formula two of Table 2;

[0034] Second, after explant disinfection and the establishment of sterile seedlings, the test-tube seedlings of Oriental fir are rapidly multiplied by tissue culture through stem section micro-cutting and bud mass propagation methods, wherein: a) micro-cutting: put the stem top of the test-tube seedling Cut the cuttings and place them on (D-2) medium: MHJ+activated carbon 3g / l, continue high-growth culture, and cut the remaining stems into small pieces of about 0.5cm and cut them into (D-1) medium: MHJ+6- BA0.5mg / l+activated carbon 3g / l induces axillary buds to germinate and pull out new shoots. After the new shoots are cut off, they are grown to a height of 5 cm and then cut into stem segments for micro-cutting propagation. The propagation ratio rea...

Embodiment 3

[0038] According to formula 3 in Table 3, the tissue culture and rapid propagation method of Oriental fir:

[0039] First, determine the formula suitable for the basic medium MHJ of Oriental fir, see formula three in Table 2;

[0040] Second, after explant disinfection and the establishment of sterile seedlings, the test-tube seedlings of Oriental fir are rapidly multiplied by tissue culture through stem section micro-cutting and bud mass propagation methods, wherein: a) micro-cutting: put the stem top of the test-tube seedling Cut off the cuttings and place them on (D-2) medium: MHJ+activated carbon 1g / l, continue high-growth culture, and cut the remaining stems into small pieces of about 0.5cm and cut them into (D-1) medium: MHJ+6- BA0.2mg / l+activated carbon 1g / l induces axillary buds to germinate and pull out new shoots. After the new shoots are cut off, they are grown and cultivated to a height of 5cm, then cut into stems and circulated for micro-cutting propagation. The p...

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Abstract

Disclosed is the fast tissue culturing and propagating technology for Taxodium mucronatum X Cryptomeria fortunei, wherein the basic cultivation medium of Taxodium mucronatum X Cryptomeria fortunei tissue cultivation comprises a large quantity of elements, ferric salts, organics, microelements and other substance, the fast tissue culturing and propagating method comprises explant sterilization, sterile sprout establishment, test tube sprout breeding, root culturing and transplanting.

Description

technical field [0001] The invention relates to a tissue culture rapid propagation technology, in particular to the tissue culture rapid propagation technology of oriental fir and the relevant formulation of the basic medium MHJ suitable for the oriental fir. Background technique [0002] Oriental fir is a new tree species obtained by the cross-genus hybridization of Chinese cedar and Mexican cypress in 1963 by Professor Ye Peizhong, a famous forest breeder in China. After decades of cutting selection and breeding, a prototype of a new tree species was formed. After the introduction in Shanghai, it was found that Oriental fir grew well in the coastal areas of Shanghai. This rare new hybrid tree species between the genera of the family Sequoia has the following characteristics: the tree shape is tall and graceful, inheriting the beautiful tree shape of Cedar and Mexican Bald Cedar; , Pond Cedar and Bald Cedar are shorter by 1-2 months, making them have a longer viewing perio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G17/00A01H4/00
Inventor 宋学孟苏敏庞瑞华王凌健陈权董举文楼文阜何燕红
Owner 上海光兆植物速生技术有限公司
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