Purified plyporus laccase and nucleic acid for coating the laccase
A laccase and coding technology, applied in the fields of nucleic acid fragments of laccase, purified enzymes, and phenol oxidase, and can solve problems such as low yields
Inactive Publication Date: 2007-08-01
NOVO NORDISK AS
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Problems solved by technology
Previous attempts to express basidiomycete laccases in recombinant hosts resulted in extremely low yields
Method used
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[0053] I. Isolation of P0lyporus pinsitus laccase
[0054] Materials and methods
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The present invention relates to isolated nucleic acid constructs containing a sequence encoding a Polyporus laccase, and the laccase proteins encoded thereby.
Description
[0001] This application is a divisional application of the application No. 95194301.4 submitted on June 15, 1995, and the invention title is "purified Polyporus laccase and nucleic acid encoding the enzyme". technical field [0002] The present invention relates to isolated nucleic acid fragments encoding a fungal oxidoreductase, and purified enzymes derived from the fragments. More particularly, the present invention relates to nucleic acid fragments encoding a phenoloxidase, in particular a laccase from Basidiomycetes-Polypores. Background technique [0003] Laccases (benzenediol:oxygen oxidoreductases) are multi-copper enzymes that catalyze the oxidation of phenolics. Oxidation of suitable phenolic substrates by laccases leads to the production of aryloxy intermediates that undergo terminal polymerization to form a mixture of dimerization, oligomerization, and polymerization reaction products. This reaction actually plays an important role in the biosynthetic pathways le...
Claims
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IPC IPC(8): C12N15/53C12N9/02C12R1/645C12N15/70C12N15/80C12N1/15C12N15/09A61K8/00A61K8/66A61Q5/10C09B53/02C12N15/00C12R1/66D06M16/00D06P1/00D06P1/32D21C5/00
CPCC12N9/0061D06M16/003D06P1/32A61K8/66D21C5/005D06P1/0048D06P1/0004C12N15/80A61Q5/10
Inventor D·S·亚夫尔F·许H·达尔伯吉P·施耐德D·A·阿斯林格
Owner NOVO NORDISK AS
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