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Buffered split flow device in use for electrophoresis of free stream

A shunt device, free technology, applied in the direction of measurement device, material analysis by electromagnetic means, analysis of materials, etc., can solve the problems of high price, less FFE enrichment, difficult research and application, etc., to simplify operation steps, reduce The effect of overall cost and cost reduction

Inactive Publication Date: 2007-08-22
SHANGHAI JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to high precision and high stability multi-channel pumps are expensive, which largely leads to high cost of FFE
However, if a general constant flow pump is used, it is difficult for the general constant flow pump to be used for high-precision and high-stability liquid delivery in FFE due to the existence of obvious unstable factors such as pressure pulses.
[0004] Second, the FFE separation mode is single
[0005] Third, FFE is rarely used for sample enrichment
This new MCRB-mediated enrichment model is difficult to complete related research and application in traditional FFE

Method used

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  • Buffered split flow device in use for electrophoresis of free stream
  • Buffered split flow device in use for electrophoresis of free stream

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Embodiment Construction

[0022] The technical solutions of the present invention will be further described below in conjunction with the accompanying drawings and embodiments.

[0023] Fig. 1 is an embodiment of the present invention - a schematic structural view of a vertical drum type buffering and diverting device. As shown in Figure 1, the buffering and diverting device of the present invention consists of a liquid storage chamber 1, an air cushion chamber 2, an input pipe 3, an input pipe interface 4, an output pipe 5, an output pipe interface 6, an air cushion regulating valve 7, an air cushion regulating syringe 8, Spirit level 9, level adjustment knob 10 and pressure gauge 11 form. The buffer chamber is composed of a liquid storage chamber 1 and an air cushion chamber 2, and a volume scale 12 is engraved on the buffer chamber. The input tube 3 is connected to the electrolyte / sample solution through the infusion pump, the input tube 3 is connected to the buffer cavity through the input tube in...

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Abstract

A buffering diverter for the free flow electrophoresis is disclosed, which is composed of a buffer cavity consisting of liquid storage chamber and air cushion chamber for buffering the pressure-flow speed pulses caused by liquid delivering pump, electrolyte / specimen solution inlet tube, air cushion regulating injector the separation chamber for free flow electrophorsis level meter and horizontal level regulating knob. The tens-hundreds stable liquid streams can be generated by its outlet tube.

Description

technical field [0001] The invention relates to a buffer splitting device for free-flow electrophoresis (Free-Flow Electrophoresis, FFE), which is used for electrolyte delivery or sample injection in free-flow electrophoresis. It belongs to the field of biochemical technology. Background technique [0002] Free-flow electrophoresis technology is a high-efficiency preparative separation technology for proteins. It uses a transverse electric field to separate and analyze proteins, viruses and cells in a continuously flowing electrolyte solution. Since the 1970s, FFE has been widely used in the isolation and analysis of genes, drugs, proteins, cells, organelles, and viruses. FFE has many advantages, such as the theoretical recovery rate is close to 100%, and it can be continuously injected for the separation of proteins / cells / viruses, etc. But there are still many problems. [0003] First, FFE is expensive. In FFE, one of the key technologies is the stable delivery of buffe...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/447C99Z99/00C07K1/26
Inventor 曹成喜武功冬王秋玲
Owner SHANGHAI JIAOTONG UNIV
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